Genome-wide identification and characterization of QTLs for transcriptional noise in human midbrain cells
Notice bibliographique
Résumé
Summary Not only the abundance of gene expression but also its cell-to-cell variation, referred to as “transcriptional noise”, is known to have certain biological significance. However, the mechanistic basis of transcriptional noise, particularly how it is regulated by genetic variants, remains elusive. In this study, we analyzed single-cell RNA sequencing (scRNA- seq) data of human induced pluripotent stem cell (iPSC)-derived midbrain cells (795,661 cells in 17 conditions) from 155 individuals with their genotypes to perform genome-wide mapping of quantitative trait loci for transcriptional noise (tnQTLs). Our analyses controlling for confounding factors such as gene expression abundance identified a total of 101,024 significant tnQTL-gene pairs. A comparison with QTLs for expression levels (i.e. eQTLs) detected by an equivalent pipeline revealed that the majority (81%) of tnQTLs were also identified as eQTLs, while no significant eQTL effects were observed in the others, and a small portion (7%) of eQTLs were with significant tnQTL effects. The tnQTLs and eQTLs showed distinctive patterns of sharing across cellular conditions, where tnQTL effects were often more condition-specific than those of eQTLs. In particular, tnQTLs without significant eQTL effects (termed tn>eQTLs) were dramatically altered by rotenone-induced oxidative stress. The tn>eQTLs also exhibited unique patterns of enrichment in various functional genomic elements, such as being frequently observed in promoters of non-QTL target genes. In the analysis using summary statistics from genome-wide association studies (GWAS) for various human complex traits, we found nominally significant enrichment of heritability for schizophrenia in tn>eQTLs. Possible contributions of tn>eQTLs to schizophrenia risk were supported by the enrichment of association signals in their target genes. We also identified genes whose transcriptional noise was implicated to be causally associated with a trait by a Mendelian randomization analysis, including HLA genes and YWHAE associated with multiple autoimmune/psychiatric disorders. To further explore the role of transcriptional noise dysregulation in disease, we analyzed scRNA-seq data from human schizophrenia and mouse model brains. Genes exhibiting differential transcriptional noise between cases and controls, i.e. differentially noisy genes (DNGs), were particularly abundant in the superficial and deep layer excitatory neurons, where association signals in schizophrenia GWAS were also enriched in their DNGs. Overall, our comprehensive mapping of tnQTLs provides a resource for a new class of regulatory genetic variants, deepens our understanding of the mechanistic basis of how genetic variants regulate transcriptional noise, and highlights the roles of tnQTLs and transcriptional noise dysregulation in human complex traits.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,001 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».