PS-12 Supplementation with selenium during pregnancy and postpartum to support ewe health
Notice bibliographique
Résumé
Abstract Selenium (Se) is an essential micronutrient known for its beneficial immunomodulatory properties. Se deficiency can increase susceptibility to pathogenic and inflammatory diseases, while also impairing fertility. Sheep raised in Se-deficient geographic regions are most vulnerable to low Se status, and this can have negative health consequences that can reduce farm productivity. Therefore, the objective of this study was to assess how Se supplementation affects the innate and adaptive immune responses of ewes consuming a Se-deficient diet. From gestation day 110 (trial day 0) to postpartum day 50 (trial day 80), 110 Dorset-Rideau ewes were fed a Se-deficient diet and supplemented daily with either 0.3 mg inorganic Se, 0.3 mg organic Se, 0.6 mg organic Se, or were not supplemented. Serum samples were obtained on trial days 0, 20, 30, 40 and 80 to evaluate Se concentrations using inductively coupled plasma mass spectrometry (ICP-MS). The Se-supplemented ewes had significantly higher serum Se concentrations compared to the non-supplemented control ewes throughout the study period (p < 0.05), and the 0.3 mg organic group had significantly higher serum Se compared to the 0.3 mg inorganic group on trial days 40 and 80 (p < 0.05). The ewes received ovalbumin (OVA) antigen immunization on trial day 0 and a second OVA immunization on trial day 10 to assess their anti-OVA IgG antibody response on trial days 0, 10 and 20 by enzyme-linked immunosorbent assay (ELISA). The antibody levels in the ewe serum significantly increased between trial days 10 and 20 (p < 0.05), but no significant treatment effects were observed on day 20. To determine how Se affected the innate immune response, an endotoxin (LPS) immune challenge was performed after weaning on trial day 80. Sera samples obtained 0 and 4 hours post-LPS injection were analyzed for cytokine (IL-6, IL-10, and IFN-γ) and chemokine (CXCL10 and CCL4) concentrations using the Luminex® 200™ platform. All treatment groups had statistically significant increases in the cytokines and chemokines, as well as rectal temperatures, from 0 to 4 hours (p < 0.05). However, no treatment-specific differences were observed. Serum cortisol concentrations were also measured 0 and 4 hours post-LPS injection by ELISA with a notable increase between hours 0 and 4 (p < 0.05), but no treatment differences were detected. Finally, serum biochemical parameters were assessed 0, 4 and 24 hours post-LPS injection to assess oxidative stress and innate immune responsivity. Se treatment had a significant effect on serum albumin, Cl, K, Na, and total protein concentrations (p < 0.05). Overall, organic Se supplementation was most effective for maintaining or increasing serum Se concentrations, however, no treatment differences in immune function were observed. This knowledge can be used by the sheep industry to optimize ewe Se-supplementation protocols.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».