1H NMR metabonomics and immune cell signature of milk may reveal insights into subclinical mastitis and quarter interdependence
Notice bibliographique
Résumé
Milk metabolome depends on a plethora of factors and on the presence of different cell types and could help understanding the biology of the mammary gland and, possibly, identifying biomarkers for mastitis, tissue repairing and milk quality. To fulfill these expectations, metabolome changes need an accurate characterization under several well-characterized physiological and pathophysiological conditions. The aim of the present work is to study mammary quarters of dairy cows affected by subclinical mastitis (SCM) and acute inflammation compared to healthy animals. The milk metabolome was investigated by 1 H-NMR spectroscopy and by the assessment of somatic cell populations by flow cytometry using a panel of leukocyte markers (CD11b, CD44, CD14, CD4, CD8, CD21). The study was integrated by microbiological evaluations and oxidized proteins (AOPP) determination and results were analyzed by multivariate model. Mammary quarters with the highest CD11b positive cells, suggestive of acute inflammation, were present in SCM-affected cows only, and were characterized by significantly higher AOPP, where the microbiological analysis revealed the presence of minor pathogens. A good PCA separation between healthy and SCM-infected animals was observed (overall error rate: 0.177±0.056) confirming that SCC are associated with modifications of milk metabolome. The classification accuracy was lower (overall error rate: 0.343±0.029) when the mammary quarters were classified on the fraction of CD11b positive cells of quarters from healthy and SCM-affected cows. Interestingly, low-CD11b-SCM samples tended to be misclassified (error rate: 0.460), suggesting the influence of infected neighboring quarters. The results of this work underlay the importance of studying the functional interdependence of mammary quarters in animals affected by SCM. Simple summary This study explored how subclinical mastitis—a mild, often hidden udder infection in dairy cows—affects the metabolites in milk. Nuclear magnetic resonance spectroscopy was used for comparing milk from healthy cows to that produced by cows with subclinical mastitis caused by bacterial infections. We found that the metabolic profile (metabolome) of analyzed milk changed noticeably in infected cows. These changes were linked to both immune cell activity and possible damage to udder tissue. Surprisingly, even parts of the udder that seemed healthy in infected cows sometimes showed altered milk composition. This suggests that infections in one part of the udder can influence nearby quarters or cause broader changes in the cow’s immune and metabolic systems. The findings support the suggestion that milk metabolites could be used to detect early stages of udder infections. The study also highlights how different quarters of the udder are connected, and how even the “healthy” ones can be affected when a cow suffers from mastitis.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».