Endometrial gene expression of lactating Holstein cows: Impact of pre-estrus progesterone and intensity of estrous expression
Notice bibliographique
Résumé
This study aimed to evaluate if different concentrations of progesterone (P4) before estrus and different intensities of estrous expression affected endometrial gene expression in lactating Holstein cows. Animals were randomly assigned into 2 experimental groups: control P4 and low P4. Cows underwent a presynchronization protocol involving a GnRH injection and P4 implant, followed by a PGF 2α injection and implant removal after 7 d, and a second GnRH injection 48 h later. The P4 treatment began 3 d after the second GnRH injection, with low P4 cows receiving 3 PGF 2α injections (12 h apart), and control P4 cows receiving no PGF 2α injections. Five days after the start of treatment, all cows underwent a hormonal protocol identical to the presynchronization, except an estradiol cypionate injection replaced the second GnRH, given 24 h after P4 implant removal. Low P4 cows received additional PGF 2α injections at 24, 36, and 48 h after the start of this second protocol. Physical activity was monitored by an activity monitor, and the duration and intensity of estrus were measured. A total of 33 cows (control P4: n=16; low P4: n=17) were submitted to uterine biopsy 7 and 14 d after the identified estrous event. Total RNA was extracted from each sample and the NanoString nCounter system was used to determine the abundance of specific mRNA molecules for 91 genes. In addition to the P4 treatment, cows were classified as high or low estrous expression based on the median estrous intensity and duration. Gene expression data were normalized and analyzed using a mixed linear regression model with cow as random intercept. Significance for differential expression was set at false discovery rate <0.1 and absolute fold change ≥1.5. The P4 treatment was not associated with changes in endometrial gene expression 7 d after estrus. However, at d 14, a total of 17 target gene transcripts were differentially expressed between the treatment groups. In low P4 cows, upregulated genes were related to the immune system ( CXCL8 , CCL22 , IL1B , IL1A , PTX3 ) and extracellular matrix remodeling ( MMP3 , MMP9 , MMP1 ), compared with control P4. Estrous expression alone did not affect gene expression. However, its interaction with the P4 treatment influenced several target genes, with most changes observed 14 d postestrus. Low P4 concentrations during the follicular growth in cows that had low intensity of estrus were linked to decreased endometrial expression of growth factors ( IGF1 , IGF2 ) and increased expression of genes related to the immune system ( CCL22 , CCL2 , CCL4 , MUC1 , NFKB2 , PTX3 ) and PGF 2α synthesis ( SLCO2A1 , PTGES ) 14 d after estrus compared control P4. Pathway analysis linked β-estradiol as a potential upstream regulator of the genes affected by the interaction between P4 and estrus. When estradiol concentrations near the onset of estrus were measured, we found that estradiol modulated endometrial expression of IGF1 and MUC1 genes, but this effect was observed only under the low P4 treatment. In conclusion, our study revealed substantial differences in endometrial gene expression between cows treated with different P4 concentrations during the follicular growth and expressing different intensities of estrus.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».