Progress, challenges and future directions in marine organic-walled dinoflagellate cyst research: New insights from an international workshop
Notice bibliographique
Résumé
Cysts are resistant life-cycle stages that play a key role in the survival and dispersal of some dinoflagellate species. Given their preservation and fossilisation potential, the organic-walled dinoflagellate cysts have been widely used as bioindicators of past and present environmental conditions. Living cysts are studied extensively due to their roles in bloom initiation, termination, and species adaptation. The use of cysts in various fields such as taxonomy, biogeography, evolution, (palaeo)ecology, and (palaeo)oceanography has expanded significantly in recent years. In this paper, we review recent developments, identify research needs, and outline future directions in marine organic-walled dinoflagellate cyst research based on round-table discussions held during the International Workshop on Dinoflagellate Cysts, which took place from 18 to 21 June 2024 in Vigo (Spain). Key priorities in taxonomy, evolution, and biogeography include the need to continue establishing connections between the cyst and motile forms along with their associated sequences, particularly for Harmful Algal Bloom (HAB) species, and updating reference databases for metabarcoding studies. Emerging molecular techniques, such as metabarcoding, provide complementary information on cyst diversity, distribution, and geographic connectivity, thereby aiding in the monitoring and reconstruction of HAB dynamics. Given the impacts of climate change on biogeographical ranges, cysts could serve as valuable indicators for tracking HAB shifts. Combining multi-omics with morphological methods could offer deeper insights into character evolution and support the construction of the dinoflagellate tree of life. Advances in the biogeochemical analysis of dinoflagellate cyst walls, particularly through the detailed study of dinosporin, are also promising for evolutionary research, as demonstrated by recent methodological advances in Fourier Transform Infrared (FTIR) and Raman spectroscopy. In palaeoceanography and palaeoecology, improving quantitative cyst-based reconstructions requires expanding the database of living cyst assemblages and their relationships with environmental variables, especially in underrepresented regions, notably in the Southern Hemisphere. Despite progress towards standardisation, there remains no universally adopted standardised methods for extracting and concentrating cysts from sediments or for quantifying cysts-essential steps for inter-site comparisons. Additionally, sediment trap studies and field observations of associated plankton are needed to complement surface sediment research and enhance our understanding of species ecology. The emerging field of palaeogenomics is promising as it complements cyst-based research. Finally, the integration of biological and geological studies to address key scientific questions is emphasised. For example, investigating the discrepancy between the accepted geological emergence of dinoflagellates and earlier suggestions from geochemistry, molecular analysis, and re-examination of acritarchs could help resolve the early phylogeny of the group.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,029 | 0,006 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,001 |
| Bibliométrie | 0,002 | 0,002 |
| Études des sciences et des technologies | 0,002 | 0,004 |
| Communication savante | 0,009 | 0,010 |
| Science ouverte | 0,002 | 0,006 |
| Intégrité de la recherche | 0,006 | 0,005 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,008 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».