METAL AND ESSENTIAL OILS-BASED ANTIBACTERIAL SOLUTIONS: TOWARDS PATIENT-SPECIFIC IMPLANTABLE DEVICES
Notice bibliographique
Résumé
Infection is among the main unmet challenges in orthopedics, causing severe societal and economical burden. Available options to prevent infection are limited and the raising incidence of microbial resistance is progressively reducing the applicability of antibiotic-eluting devices. Here we show two different approaches to functionalize implantable devices, without using antibiotics. One route exploits the deposition, by plasma-assisted techniques (Ionized Jet Deposition and Femto-Second Pulsed Laser Deposition) of metal-based coatings (silver, copper and zinc-based) on the prostheses surface. The second route is based on essential oils. Both approaches are also used to obtain antibacterial patches, manufactured by electrospinning, to be used alone or in combination with prostheses. Metal coatings composition (XRD), morphology and thickness (FEG-SEM, AFM), and biocompatibility (Alamar Blue test, fibroblasts) are measured, depending on deposition parameters. Then, antibacterial efficacy is measured on S. Aureus, E. Coli, E. Faecalis, and P. Aeruginosa, in terms of planktonic growth, adhesion and biofilm formation, exploiting the Calgary Biofilm Device. Our results show that coatings have tunable characteristics (thickness, surface roughness), and can be applied to a variety of substrates (ceramic, metallic and polymeric), including custom-made prostheses, with high reproducibility, adhesion to substrate and no defects. They are biocompatible and can inhibit planktonic and biofilm growth of gram + and gram – strains. Antimicrobial efficacy is strain-dependent, but complete inhibition can be achieved by tuning the films characteristics. These results suggest that selecting a metal based on microbial contamination would be a more promising approach compared to the sole use of silver, moving towards a personalized medicine in infection. For essential oils-based patches, we started from a screening of the antibacterial oils used in the literature, including the non-medical field. The selected oils (thymol, lavender, turmeric, ginger, lemon, cinnamon, citronella, tea tree, black pepper) are screened and those having the lowest MIC are selected and used for electrospinning (using PCL and PEO as a matrix). After electrospinning the patches, we measured their stability, morphology, antibacterial activity (against S. Aureus and E. Coli) and biocompatibility. Our results show that all oils can be effectively spun and are retained in the patch, thus providing high antibacterial action. The antibacterial activity depends on the oil and the polymeric matrix, with tea tree oil and black pepper being the most effective for the strains under examination. As for the metals, higher efficacy can be achieved by a proper selection of single/combined oils to address specific bacterial strains.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».