PSVI-11 Role of differentially expressed and imprinted genes in high and low fertility produced cattle pregnancies.
Notice bibliographique
Résumé
Abstract Pregnancy loss is considered one of the main causes of reproductive inefficiency in beef cattle. Most research focuses on female contribution to pregnancy success leaving the role of the sire in pregnancy loss largely understudied. The objective was to determine the differentially expressed genes (DEGs) and imprinted genes between the conceptus and uterus of pregnancies produced by sires of known fertility. We hypothesized that the paternal genome would have a significant effect on transcript abundance between these tissues. The sires used have been previously reported to have significantly different levels of field fertility; however, they had passed all normal quality control standards for frozen-thawed semen. Bos indicus heifers (n=45) were subjected to estrus synchronization and embryo transfer with IVP embryos from either a High Fertility (HF) or a Low Fertility sire (LF), pregnancies were confirmed at slaughter. Samples were collected from the trophectoderm (TE) and caruncle (CAR) on days 25 and 36 of gestation for transcriptome analysis. Total RNA was isolated from tissue samples using the RNeasy kit (QIAGEN; Hilden, Germany) per manufacturer’s instructions. Sequencing was conducted using an Illumina platform. Sequences were aligned to the reference genome ARS-UCD1.2. DEGs between sires and tissue were determined using edge-R package from R. False discovery rate was 0.1. On day 25, on the TE 29 DEGs and 42 imprinted genes were identified. In the CAR, 16 DEGs and 43 imprinted genes were observed. On day 36 in the TE, 73 DEGs and 46 imprinted were identified. In CAR, there were 40 DEGs and 33 imprinted genes. Gene ontology analysis reported DEGs in the LF compared to the HF were associated with immunology and reproduction. Downregulated DEGs of particular interest: BoLA NC-1 known to be expressed by the trophoblast cells to inhibit the activation of uterine leukocytes and to protect the conceptus from immune-mediated rejection. The APOB gene, known for its involvement in embryonic development, spermatogenesis and flagellated sperm motility, as well as FETUB known for its function of binding sperm to the zona pellucida. Imprinted genes of interest included OOEP known for embryonic pattern specification, establishment of apical/basal cell polarity. MEAK7 involved in TOR signaling; positive regulation of protein localization to lysosome; and response to insulin. Lastly, PHLDA2 known for its role in placental development, regulation of gene expression and embryonic development. These data suggest that field fertility records might be inaccurate for bull selection to improve reproductive performance. The downregulation of these genes appears to be detrimental to pregnancy success and could be responsible for variation seen in conception rates. Results from this study could lead us to identify sub-fertile bulls. It is evident that the paternal genome seems to play a much bigger role in pregnancy establishment than originally thought.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».