MON-191 In Vivo Overexposure to Non-esterified Fatty Acids Increases Circulating Androgen Levels in Normal Mongrel Dogs
Notice bibliographique
Résumé
Abstract Disclosure: C. Richer dit Laflèche: None. J. Faubert: None. F. Naimi: None. S. Bellanger: None. M. Battista: None. O. Benrezzak: None. A. Carpentier: None. J. Baillargeon: None. Background. The exact etiology of polycystic ovary syndrome is poorly understood, but studies suggest that lipotoxicity may be an important contributor. The aim of this study was to evaluate the effects of an in vivo overexposure to non-esterified fatty acids (NEFA) on changes in circulating androgen levels in a dog model, which was selected for its similarities with humans in steroidogenesis and energy metabolism. Methods. Female mongrel dogs (27.6 ± 2.9 kg) underwent a 17.5-hour metabolic protocol. During the first 8 hours (night period), dogs (n=6/group) received either a Intralipid-heparin 20% (IH; 0.02 ml/kg/min+0.5 U/kg/min) or a saline infusion (control group), followed by a standard meal at 8:30. Blood samples were drawn every 60 minutes during the night period (8h) and for 9 hrs post-meal (day period). Only 2 dogs (1/group) were in estrus. Changes from baseline were calculated as the ratio of mean levels during the period to the level measured at baseline (before IH infusion initiation) and compared between groups using Wilcoxon tests. Associations of androgen (testosterone, androstenedione, 17-hydroxyprogesterone (17-OHP), and DHEA) ratios with insulin and NEFA ratios during each test phase were tested using Spearman’s correlations. In addition, NEFA or insulin ratios were correlated with 17-OHP ratios corrected for potential confounders (individually, by dividing 17-OHP ratios by either cortisol, ACTH, insulin or NEFA ratios). Results. Baseline androgen levels were comparable between experimental groups. NEFA levels nearly doubled during the nocturnal IH infusion compared with the saline group (1.77 vs. 0.93-fold increase, p=0.015) and rapidly returned to normal after discontinuation. 17-OHP level ratios were significantly increased 1.5-fold in the IH group compared to the saline group both at night (0.86 vs. 0.56, p=0.026) and more than doubled during the day (0.90 vs. 0.42, p=0.009), without significant changes in other androgens. Nocturnal NEFA ratios correlated with both the nocturnal and diurnal 17-OHP ratios (rho=0.783, p=0.003; and rho=0.755, p=0.005); and diurnal insulin ratios correlated with 17-OHP ratios (rho=0.615, p=0.03). Correcting for nocturnal insulin ratios weakened the correlation between nocturnal 17-OHP ratios and NEFA ratios (rho=0.434, p=0.159), indicating a confounding effect. Moreover, adjusting for diurnal insulin ratios decreased the correlation between diurnal 17-OHP ratios and nocturnal NEFA ratios (rho=0.378, p=0.226). The association between diurnal 17-OHP ratios and diurnal insulin ratios was partly confounded by both night and day NEFA ratios (rho=0.336; and rho=0.224). Associations were mostly independent of ACTH and cortisol ratios. Conclusion. An acute in vivo elevation of NEFA using an IH protocol induces a significant increase in androgen production through a mechanism that is partially dependent on insulin. Presentation: Monday, July 14, 2025
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».