Evaluation of Discoidin domain receptor 1 (DDR1) in junctional epithelial permeability and wound healing
Notice bibliographique
Résumé
Abstract Background Epithelium is the periodontal first line of defense against microbes. Discoidin domain receptor 1 (DDR1) is a collagen receptor expressed in epithelium. Ddr1 knockout ( Ddr1 −/− ) mice develop periodontitis‐like defects, including junctional epithelium (JE) downgrowth, bacterial invasion, and alveolar bone loss. The objective of this study was to investigate epithelial responses in the absence of DDR1. We hypothesized that Ddr1 −/− mice exhibit increased JE permeability and delayed epithelial wound healing. Methods Epithelium was analyzed in Ddr1 −/− and wild‐type ( Ddr1 +/+ ) mice. JE permeability was studied in vivo by applying a fluorescent dye and measuring dye penetration. Immunohistochemistry (IHC) was used to identify the localization of E‐cadherin and collagens IV, VIII, and XVII in oral epithelium. DDR1 expression in wound healing was confirmed by histology. Epithelial wound healing responses were assessed by creating skin and oral wounds and measuring epithelial migration and wound closure. Results No differences in JE permeability were observed between Ddr1 −/− and Ddr1 +/+ mice, although a trend in the means was observed toward decreased dye surface area ( p = 0.07) and intensity ( p = 0.08–0.09) in the periodontium of the former mice. IHC did not reveal differences in the localization of E‐cadherin or collagens IV, VIII, and XVII between genotypes. In human gingiva, DDR1 was expressed at the epithelial front, migrating to cover palatal wounds. Wound healing experiments revealed a higher % wound healing of dorsal skin in Ddr1 −/− than Ddr1 +/+ mice at 5 days post‐wounding (dpw) ( p = 0.01). Conclusions DDR1 does not affect JE permeability but may play a role in effective epithelial cell migration during cutaneous wound healing. Plain language summary Epithelium is the periodontal first line of defense against microbial attacks. Discoidin domain receptor 1 (DDR1) is a collagen receptor expressed at the epithelium. Mice not expressing the receptor ( Ddr1 −/− mice) develop defects consistent with periodontitis, including epithelium downgrowth and bone loss. In this study, we investigated periodontal epithelial permeability by applying a fluorescent dye in the mouth of Ddr1 +/+ and Ddr1 −/− mice. Additionally, we used histological methods to reveal differences in the localization of gingival proteins between Ddr1 +/+ and Ddr1 −/− . Finally, we investigated the role of DDR1 in wound healing in human sections and in a live animal model. No differences in junctional epithelium (JE) permeability were observed between Ddr1 +/+ and Ddr1 −/− mice, as expressed by the comparable presence of dye in the periodontal tissues of both types of mice. There were no differences in the localization of E‐cadherin or collagens IV, VIII, and XVII between Ddr1 +/+ and Ddr1 −/− . In human gingiva, DDR1 was expressed at the epithelial front, migrating to cover palatal wounds. The animal wound healing study revealed higher healing of skin wounds in Ddr1 −/− than Ddr1 +/+ mice at 5 dpw. In conclusion, this study has elucidated a role for DDR1 in epithelial cell migration during skin wound healing.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».