In vitro anti-diabetic activity and molecular docking studies of Amaranthus cruentus, Amaranthus hybridus and isolated compounds
Notice bibliographique
Résumé
BACKGROUND: Amaranthus species are traditional leafy vegetable also reputed for their potential in managing metabolic disorders, including diabetes. In previous investigations conducted by our group, extracts from Amaranthus cruentus and Amaranthus hybridus, along with isolated compounds, exhibited strong α-glucosidase enzyme and moderate α-amylase inhibition in vitro. METHODS: The present study evaluated the antidiabetic effects of extracts and isolated compounds on C3A hepatocytes and L6 myoblasts. In addition, to elucidate the potential molecular mechanisms behind the observed enzymatic inhibition, we conducted docking studies of the isolated compounds against α-glucosidase and α-amylase. The finely powdered leaf samples were mixed with methanol and extracted using a cold maceration process. Three compounds were obtained from the aerial sections of the plant material using silica gel chromatography. Glucose metabolism of the extracts and the isolated compounds was carried out using L6 myoblasts and C3A cells. RESULTS: The plant extracts of A. cruentus and A. hybridus and isolated compounds; palmitic acid and α-spinasterol, were found to stimulate glucose uptake in L6 myoblasts and C3A cells at 31.25 µg/mL, A. cruentus and A. hybridus stimulated glucose uptake by 15.67% and 15.95%, respectively, while insulin (10 µg/mL) boosted glucose uptake by 27.1% in L6 myoblasts, although their mechanisms of action remained unclear. Palmitic acid and α-spinasterol enhanced glucose uptake in L6 myoblasts by 28.88% and 21.61%, respectively. After 48 h of pre-treatment, only α-spinasterol showed a significant increase in glucose absorption (56.82%) in L6 myoblasts at a treatment dose of 62.5 µg/mL, while insulin showed a glucose uptake of 78.02%. A. hybridus demonstrated significant glucose uptake (20.86%) in C3A hepatocytes after 24 h of pre-treatment at a dosage of 15.63 µg/mL, in contrast to insulin, which enhanced glucose uptake by 34.8% at 10 µg/mL. Compared to acarbose with a binding affinity of -7.7 Kcal/mol, palmitic acid, α-spinasterol and pheophorbide A-methyl ester showed the binding affinities of -5.7 Kcal/mol, -7.6 Kcal/mol and - 7.8 Kcal/mol respectively, on α-glucosidase. Acarbose showed a comparable binding affinity of -6 Kcal/mol to those of pheophorbide A-methyl ester and α-spinasterol, which showed the binding scores of -6.7 Kcal/mol and - 6.8 Kcal/mol, respectively. CONCLUSION: This study validates A. cruentus and A. hybridus's traditional use for the treatment of diabetes mellitus. Future studies will investigate the underlying mechanisms by which the extracts and compounds enhance glucose uptake in L6 myoblasts and C3A hepatocytes. Additionally, to provide a more comprehensive understanding of the antidiabetic potential of the Amaranthus extracts and compounds, in vivo experiments using appropriate animal models will be conducted.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».