Understanding biofilm development on diverse interfaces and controlled environments using novel microfluidic devices
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Notice bibliographique
Résumé
Biofilms are ubiquitous structured communities of bacterial cells encased in a selfproduced polymeric matrix that protects the microorganisms from hostile physical and chemical environments that grow and respond to stimuli from internal biological processes and external environmental conditions. Biofilms can attach to different surface types and could be beneficial or detrimental depending on the microbial species and their growth. Among all the environmental factors, the interfaces in which biofilm grows play a critical role that directly affects how they thrive and interact with the environment. While there are many devices available to culture biofilm for <i>in vitro </i>studies, such as the microtiter plate, the Calgary Biofilm Device, the Center for Disease Control reactor, and the rotating disk reactor, most of these platforms are somewhat limited to the modelling of solid-liquid interface (SLI) biofilms, where the biofilms are attached to solid-rigid platforms submerged in liquid. Indeed, the environment in which biofilms thrive is diverse, and they could grow on air-liquid interface (ALI), liquidliquid interface (LLI), solid-air interface (SAI) <i>etc</i>. For example, biofilms found along the respiratory tracts and on the roots or leaves of plants are ALI biofilms, whereas biofilms in the urinary tract and on porous membranes that separate different media, such as those commonly found in wastewater treatment, can be best represented as LLI biofilm. Despite the plethora of scientific studies on biofilm, a detailed description of how biofilm develops on different interfaces in a well-controlled environment remains scarce. Being able to control the environment is important to meaningfully compare the different properties and growth patterns exhibited by the biofilm models developed on different interfaces. A device with an inclusive platform that enables the change in interfaces while allowing for the precise manipulation of growth conditions and environmental factors would also be useful and important to determine the efficacy of biofilm control methods accurately.The studies in this thesis have been broadly categorised into four sections, specifically: (1) the design and development of a microfluidic device that enables the use of different interfaces for biofilm culture, (2) the application of the microfluidic device for the dynamic control of environmental conditions to understand biofilm growth on different interfaces, (3) the design and development of a device that enables the real-time <i>in situ </i>electrochemical monitoring of biofilm growth, and (4) demonstration on how the aforementioned devices can be used to access the efficacy of novel drug formulations for treating biofilm.
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Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découle