Effects of a fecal transplant from anxious donors on rehabilitative training, microbiota composition, systemic inflammation and behaviour following a unilateral cervical spinal contusion (C5, 125kdyn) in female Lewis rats
Notice bibliographique
Résumé
STUDY PURPOSE: The purpose of this study was to determine whether optimal donor selection would influence the outcome of a fecal microbiota transplant (FMT) and the efficacy of rehabilitative training after a unilateral C5 spinal contusion (infinite horizons impactor, 125 kdyns) in female Lewis rats. Uninjured, genetically identical FMT donors (n=10) were selected as rats who displayed naturally reduced baseline activity levels and increased anxiety-like behaviour. Experimental groups consisted of a group that was gavaged with the FMT solution for 3 days following SCI (SCI+FMT, n=15) and a group that received a vehicle control solution (SCI+Vehicle, n=15). DATA COLLECTED: Data collected for this study include: 16s rRNA sequencing of fecal matter collected pre-injury, on the day of injury, then 3, 7, 14 and 56 days post injury. This data includes the bacteria bacteroidetes, firmicutes, proteobacteria, cyanobacteria, lactobacillus, as well as the alpha diversity and genus information. Rehabilitation training on a single pellet reaching task. This includes the number of attempts and success rate at baseline (pre-injury) and throughout 8 weeks of rehab training after SCI (10 min per day, 5 days per week, analyzed once per week). Von Frey test at 1 and 9 weeks post SCI (normalized to baseline values). The percentage of ipsilesional paw placements in the cylinder test (9 weeks post SCI). The total distance travelled in the open field (9 weeks post SCI). The total distance travelled and the percentage of time spent in the open arms of an elevated plus maze (9 weeks post SCI). The time spent in the light component of the light-dark box test (9 weeks post SCI). The percentage of sucrose solution consumed over 2 hours (9 weeks post SCI). The time spent interacting in the social interaction test (9 weeks post SCI). Lesion analysis. IBA1 density immediately caudal to the lesion, at and immediately rostral to the lesion. Plasma analytes were measured at baseline, 3, 21 and 77 days post SCI. FITC dextran assay for intestinal permeability: This was a separate experiment, re-using the animals from the uninjured group after all of their behavioural testing was complete. This data shows plasma FITC concentrations 7 days post SCI displayed as a fold change from baseline. Lesion analysis and plasma analytes at 7 days post SCI are also included for these animals. DATA USAGE NOTES: The data presented in this dataset show that a fecal transplant from anxious donors (with decreased levels of Lactobacillus in their stool) does not prevent SCI-induced dysbiosis. This fecal transplant had some long-term side effects on systemic and local inflammation and also increased anxiety-like behaviour in the recipient rats. Overall, this study shows that optimal donor selection is critical for successful FMT treatment following SCI.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,001 |
| Méta-épidémiologie (sens large) | 0,002 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».