Study on Metabolism of Natural Medical Components In Vivo: Metabolism Study in Rat After Oral Administration of Rhubarb Decoction and Characterization, Identification of the Rat Metabolite of Scutellaria baicalensis
Notice bibliographique
Résumé
Recent Advances in Theories and Practice of Chinese Medicine 408 pharmacokinetic study of rhubarb.Total of 39 compounds were identified as absorbed bioactive constituents.The excretion of M22 and M39 reached more than 1000 μg in urine samples after intragastric administration of the decoction. Experiments Chemicals and materialsStandards of the rhapontin, aloe-emodin, chrysophanol, emodin, rheochrysidin and rhein were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP) (Beijing, China).The plasma, urine and tissue samples were extracted using Supelco TM LC-18 solid-phase extraction (SPE) tubes (1ml/100mg, USA).Acetonitrile (HPLC grade) was purchased from the Dikma Company (Dikma, USA).Water was deionized and double distilled.All other chemicals were of analytical grade.Blank rat plasma (drug free) was prepared in our laboratory. EquipmentAn HPLC-MS system (Agilent 1100, Agilent Technologies, Wilmington, DE, USA) with an electrospray ionization (ESI) ion source was used.The LC/MSD Trap software (version 5.3) was used for data acquisition and processing.An API 4000 triple quadrapole instrument (Applied Biosystems, Toronto, Canada) was used for the mass spectrometric detection using an electrospray ionization (ESI) source in the negative mode.A high-speed bench-top centrifuge (Sorvall ST16, Thermo Fisher Scientific, Germany) was used for centrifuge biological samples. Preparation of rhubarb decoction200 g rhubarb was immerged in 2000 mL distilled water and then refluxed for 1 h at 100ºC.The aqueous extract was filtered and the residue was refluxed for 30 min with another 2000 mL of distilled water under the same conditions.The two water extracts were combined and condensed to 200 mL and stored at 4ºC until use. Standard preparationThe standard of rhapontin, aloe-emodin, chrysophanol, emodin, rheochrysidin and rhein were dissolved in methanol at a concentration of 100 μg/ml to obtain the standard stock solutions. Animals30 male Sprague-Dawley rats (200±20 g) were obtained from the Animal Center of Shanghai Institute of Materia Medica, Shanghai, China.They were kept under standard laboratory conditions (12/12 h light/darkness, 22±2ºC room temperature, 50-60% humidity) for one week prior to the experiments.All animal experiments were carried out according to the Guidelines for the Care and Use of Laboratory Animals, and were approved by the Animal Ethics Committee of Shanghai Institute of Materia Medica.Six male rats were orally administered a single dose of rhubarb decoction at 10 g/kg.After dosing, the rats were housed in separate glass metabolic cages with free access to water.Urine was collected at -8 to 0 h predose and during 0-2, 2-4, 4-6, 6-8, 8-24, 24-36, 36-48 h postdose.www.intechopen.com
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,003 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,001 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».