Additional file 1 of mTORC1-mediated acquisition of reward-related representations by hippocampal somatostatin interneurons

Additional file 1: Figure S1. Inhibition of mTORC1 signaling in SOM-INs in SOM-Rptor-KO mice and other behavioral measures during learning. A Representative confocal immunofluorescence labelling of S6 phosphorylation in EYFP-expressing SOM-INs. Repeated mGluR1 stimulation increased p-S6 in SOM-INs relative to sham-treatment in slices from control SOM-IRES-Cre mice but not in slices from SOM-Rptor-KO mice. Scale bar: 20 µm. B Quantification of p-S6 immunofluorescence showing reduced basal level of p-S6 in SOM-INs of SOM-Rptor-KO mice relative to control SOM-IRES-Cre mice suggesting deficit of constitutive mTORC1 activity. C Quantification of evoked p-S6 showing increased p-S6 in SOM-INs after repeated mGluR1 stimulation relative to sham treatment of SOM-IRES-Cre mice but not in SOM-Rptor-KO mice confirming a deficit in mTORC1 signaling in SOM-INs of SOM-Rptor-KO mice. D, E Summary plots of changes over training sessions in SOM-IRES-Cre and SOM-Rptor-KO mice showing similar reduction in trial duration over training in both mice, and increase in percentage time spent in reward zone over training only in control SOM-IRES-Cre mice, indicative of a spatial learning deficit in SOM-Rptor-KO mice. F Summary plot of learning index measured for the same number of trials for each animal in the last training session, indicating a learning deficit in the SOM-Rptor-KO relative to control mice when trial numbers are constant. Details of statistical tests provided in Additional file 5: Table S1. * p < 0.05, *** p < 0.001, ns not significant.

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