Probiotic treatment in female Lewis rats following unilateral incomplete cervical (C5) contusion injury: behavior, histological and systemic cytokine data
Notice bibliographique
Résumé
STUDY PURPOSE: We tested whether a probiotic treatment immediately after a unilateral incomplete cervical spinal cord injury in female rats prevents gut dysbiosis, promotes motor recovery and dampens the related anxiety like behavior as we have previously reported for fecal matter transplants. DATA COLLECTED: Female Lewis rats (12 weeks and 180-220 g) received a 125 kdyn unilateral contusion on the right side of the spinal C4-5 level (1.25 mm right of midline, 25 mm drop height at a 15-degree angle) using an Infinite Horizon impactor. Probiotics were given immediately after SCI using VSL#3 containing eight different strains (Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus planatarum, and Streptococcus salivarus subspecies thermophilus). VSL#3 was administered daily via oral gavage for 7 days at a dose of 5 billion CFU. Control group was gavaged with 0.5 ml of sterile water. After 7 DPI, the 5 billion CFU of VSL#3 doses were adjusted to ensure equivalent consumption and administered ad libitum in drinking water for a total of 35 days post injury (DPI). An open field test was used to assess motor function as well as depression/anxiety-like behavior. The elevated plus maze was carried out at baseline and 35 DPI by recording the rats for ten minutes and then analyze offline with customized software (https://github.com/cdoolin/rat-apps). The rats were placed in a cylinder for 5 min to record the number of left and right forelimb paw placements on the cylinder wall during rearing and recorded weekly after injury. For the light-dark exploration test, the distance traveled in the light chamber, total number of transitions, time spent in each chamber and latency to enter the light chamber were recorded and analyzed offline. The test was carried out at baseline and 35 DPI. The horizontal ladder served to examine skilled motor function, where a successful trial was concluded when rats walked across all rungs without rearing or stopping. Three successful trials were analyzed offline for the number of successful steps, missed steps, and forelimb slips.The von Frey test was used to assess mechanical allodynia. The overall gut microbiome was assessed prior to injury (baseline) and at 3, 7, 14, and 35 DPI by collecting fresh fecal matter from rats at the beginning of their dark cycle and submitted for 16S rRNA analysis. The microbiome report for this study is in the associated odc-sci dataset #688. Plasma samples were collected in the morning (animal's light cycle) and diluted 2-fold and run on the Rat Cytokine 27-Plex and Rat Stress Hormone 2-Plex discovery assays. Images of lesion extension were taken from cryosectioned tissue slices with an epifluorescence microscope at 5× magnification and analyzed using ImageJ. Lesion size was calculated and represented as the percent of spared tissue. Images at 5× magnification were taken to visualize the entire spinal cord cross section 0.25 cm rostral to the lesion, at its epicenter, and 0.25 cm caudal. To analyze microglia in the spinal cord tissue, the area of Iba-1+ immunoreactivity was divided by the total area of each individual spinal cord cross section and expressed as a percentage of Iba1+ area using thresholding. DATA USAGE NOTES:
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,001 |
| Méta-épidémiologie (sens large) | 0,002 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,002 | 0,003 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,004 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; les deux têtes enseignantes s’accordent sur ce qui est montré ici.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».