Additional file 2 of Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury
Notice bibliographique
Résumé
Additional file 2.Fig. S1. Cytokine profiling in conditioned media produced by potential COVID-19 cell products. Representative images of the proteome array used to analyze conditioned media produced by BM-MSCs, HDCs, or UC-MSCs. The table on the right panel lists the position coordinates of cytokine antibody on the array.Fig. S2 Relative cytokine content within HDC conditioned media compared to BM-MSC conditioned media. Expression of cytokines within conditioned media produced by HDCs compared to BM-MSCs. Data were compared using an unpaired t-test with individual variances for samples and a two-stage step-up (Benjamini, Krieger, and Yekutieli) false discovery rate to account for multiple comparisons. All data are presented as individual and mean values ± SEM, n=3 biological replicate; each circle represents one data point from one unique biological replicate. Significance is indicated on the panels. Fig. S3. Relative cytokine content within UC-MSC conditioned media compared to BM-MSC conditioned media. Expression of cytokines within conditioned media produced by UC-MSCs compared to BM-MSCs. Data were compared using an unpaired t-test with individual variances for samples and a two-stage step-up (Benjamini, Krieger, and Yekutieli) false discovery rate to account for multiple comparisons. All data are presented as individual and mean values ± SEM, n=3 biological replicates; each circle represents one data point from one unique biological replicate. Significance is indicated on the panels.Fig. S4. Relative cytokine content within UC-MSC conditioned media compared to HDC conditioned media. Expression of cytokines within conditioned media produced by UC-MSCs compared to HDCs. Data were compared using an unpaired t-test with individual variances for samples and a two-stage step-up (Benjamini, Krieger, and Yekutieli) false discovery rate to account for multiple comparisons. All data are presented as individual and mean values ± SEM with n=3 biological replicates; each circle represents one data point from one unique biological replicate. Significance is indicated on the panels. Fig. S5. Cytokine concentrations found within the lung of critically ill COVID-19 patients. This figure depicts literature search results of cytokine concentrations found in bronchial alveolar lavage fluid from critically ill patients with COVID-19. When data were not presented in a tabular form, they were extracted from the figures by using online tool WebPlot Digitizer. All data are presented as individual and mean values ± SEM. The values on the bar graphs represent arithmetic mean of all studies for respective cytokine. Fig. S6. Flow cytometry showing co-segregation of receptors for COVID-19 related cytokines. Representative flow cytometry images demonstrating detection of FITC anti-human IL-1R1, BV421 anti-human IL-2Rβ, PerCP-eFluor710 anti-human IL-6R, PerCP-cy5.5 anti-human IL-8RA, APC anti-human IL-10R, and PE anti-human TNFR1on BM-MSCs, HDCs and UC-MSCs. Isotype controls were used to correct compensation and confirm antibody specificity.Fig. S7. COVID-19 ARDS cytokine challenged UC-MSC conditioned media effect on pulmonary microvascular endothelial cell permeability. Endothelial cell permeability assay showed that treating pulmonary microvascular endothelial cells with conditioned media (CM) collected from COVID-19 ARDS cytokine cocktail challenged UC-MSCs does not alter endothelial cell permeability. Filled circles represent cytokine cocktail treatment, Hollow circles represent no cytokine cocktail treatment. All data are presented as individual and mean values ± SEM. n=3 biological replicates; each circle represents one data point from one unique biological replicate.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,003 | 0,038 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,001 |
| Méta-épidémiologie (sens large) | 0,002 | 0,001 |
| Bibliométrie | 0,002 | 0,003 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,003 | 0,002 |
| Science ouverte | 0,002 | 0,001 |
| Intégrité de la recherche | 0,002 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,892 | 0,168 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».