Additional file 4 of Reduced microglia activation following metformin administration or microglia ablation is sufficient to prevent functional deficits in a mouse model of neonatal stroke
Notice bibliographique
Résumé
Additional file 4: Figure S4. Dose-dependentmicroglia ablation is seen following one week of daily injections of Plexxikon.A Experimental timeline following PLX 5622 administration (vehicle control,10ug/g or 50ug/g) from P8 to P15. Immunohistochemistry (IHC) or the neurosphereassay (NS) was performed on P15. B No differences in pup weight were recordedacross treatment groups. C Representative image of microglia (Iba1+, red) atP15 in the cortex in Sham mice (20x magnification). D Representative image ofmicroglia (Iba1+, red) at P15 in the cortex in mice that received 10ug PLX fromP8 to 15 (20x magnification). E Representative image of microglia (Iba1+, red)at P15 in the cortex in mice that received 50ug PLX from P8 to 15 (20xmagnification). F Quantification of the number microglia (Iba1+ cells/area) inthe cortex, reported as fold change. Microglia are significantly depleted with10ug/g PLX (1.00±0.11-fold change of Iba1+ cells in vehicle-treated mice vs.0.52±0.03-fold change Iba1+ cells in 10ug/g PLX-treated mice) (p=0.008) and50ug/g PLX (1.00±0.11-fold change of Iba1+ cells in vehicle-treated mice vs.0.18± 0.05-fold change of Iba1+ cells in 50ug/g PLX-treated mice (p=0.0004).PLX at 50ug/g results in a significantly greater loss of Iba1+ cells comparedto 10ug/g (0.52±0.03-fold change Iba1+ cells in 10ug/g PLX-treated mice vs.0.18±0.05-fold change of Iba1+ cells in 50ug/g PLX-treated mice) (p=0.033).Average number of Iba1+cells/unit area: Vehicle=67.17±7.21; 10ug/gPLX=35.28±2.29; 50ug/g PLX=12.42±3.2. G Representative image of microglia(Iba1+, red) at P15 in the striatum in Sham mice (20x magnification). HRepresentative image of microglia (Iba1+, red) at P15 in the striatum in micethat received 10ug PLX from P8 to 15 (20x magnification). I Representativeimage of microglia (Iba1+, red) at P15 in the striatum in mice that received50ug PLX from P8 to 15 (20x magnification). J Quantification of the numbermicroglia (Iba1+ cells/area) in the striatum, reported as fold change.Microglia are not depleted with PLX at 10ug/g (1.00± 0.08-fold change of Iba1+cells in vehicle-treated mice vs. 0.77±0.08-fold change Iba1+ cells in 10ug/gPLX-treated mice) (p=0.4514) but are significantly reduced with 50ug/g PLX(1.00± 0.08-fold change of Iba1+ cells in vehicle-treated mice vs.0.25±0.04-fold change of Iba1+ cells area in 50ug/g PLX-treated mice vs.vehicle (p=0.003). PLX at 50ug/g results in a significantly greater loss ofIba1+ cells compared to 10ug/g (0.77±0.08-fold change Iba1+ cells in 10ug/gPLX-treated mice vs. 0.25±0.04-fold change of Iba1+ cells in 50ug/g PLX-treatedmice) (p=0.0034). Average number of Iba1+cells/unit area: Vehicle = 32.19±2.65;10ug/g = 28.00 ± 2.65; 50ug/g PLX = 9.83±1.36. K Representative image ofmicroglia (Iba1+, red) at P15 in the SEZ in Sham mice (20x magnification). LRepresentative image of microglia (Iba1+, red) at P15 in the SEZ in mice thatreceived 10ug PLX from P8 to 15 (20x magnification). M Representative image ofmicroglia (Iba1+, red) at P15 in the SEZ in mice that received 50ug PLX from P8to 15 (20x magnification). N Quantification of the number microglia (Iba1+cells/ area) in the SEZ, reported as fold change. Microglia are significantlydepleted with 10ug/g PLX (1.00± 0.04-fold change of Iba1+ cells invehicle-treated mice vs. 0.55±0.03-fold change Iba1+ cells in 10ug/gPLX-treated mice) (p=0.0002) and 50ug/g PLX (0.31±0.01-fold change of Iba1+cells in vehicle-treated mice vs. 0.18±0.05-fold change of Iba1+ cells in50ug/g PLX-treated mice (p=0.004). PLX at 50ug/g results in a significantlygreater loss of Iba1+ cells compared to 10ug/g (0.55±0.03-fold change Iba1+cells in 10ug/g PLX-treated mice vs. 0.31±0.01fold change of Iba1+ cells in50ug/g PLX-treated mice) (p=0.0038). Average number of Iba1+cells/unit area:Vehicle=20.03±0.87; 10ug/g PLX=11.11±0.68; 50ug/g PLX=6.19±0.10±1.36. O Nodifference in neurosphere numbers were observed across treatment groups(p=0.39). Average number of neurospheres: Vehicle = 6.33±0.96; PLX: 7.33±0.42. n=3 mice per group. Data presented as mean ± SEM. Unit area (cortex, striatum):3x0.105 mm2. Unit area (SEZ): 3x0.045mm2. Statistics: (B) Two-way ANOVA; (C-E)One-way ANOVA. (O) Unpaired t-test. *p<0.050.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,002 | 0,015 |
| Méta-épidémiologie (sens strict) | 0,002 | 0,001 |
| Méta-épidémiologie (sens large) | 0,002 | 0,001 |
| Bibliométrie | 0,002 | 0,003 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,002 | 0,002 |
| Science ouverte | 0,002 | 0,001 |
| Intégrité de la recherche | 0,002 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,876 | 0,132 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».