Additional file 1 of Prohibitin 1 interacts with p53 in the regulation of mitochondrial dynamics and chemoresistance in gynecologic cancers
Notice bibliographique
Résumé
Additional file 1 Figure S1. Protein-protein interaction analysis by PLA. Control of OVCA and CECA cell and description of Duo link PLA count method. (A) A2780s (ovarian cancer cells- OVCA), (B) C13 (cervical cancer cells – CECA), and (C) human ovarian tumour section without treatment of PLA reagents (contro. Blue represents DAPI (nucleus marker) and green represents TOM20 (mitochondria marker). (D) OV2008 cells treated without (CTL) or with CDDP were subjected to PLA assay. Using Duolink image tool, white dots (PLA signal) were counted either in mitochondria (green) or nucleus (Blue). Cell number was automatically assigned as shown by Duolink image tool. (E) PLA signal in each cell were counted, summed and averaged by number of cells as shown in table. Figure S2. CDDP increased Phb1 content and apoptosis in OV2008 cells, but not in C13* cells. (A) and (B) Comparison of Phb1 contents and apoptosis in OV2008 and C13* cultured with CDDP at different concentrations (A: 0–10 μM, 24 h) or for different duration (B: 0–24 h, 10 μM). Contents of Phb1 and GAPDH (loading control) were examined by Western blotting. Apoptosis was examined by Hoechst assay. Phb1 contents in OV2008 cells but not in C13* cells were significantly increased in the presence of CDDP in a concentration- (A; **p < 0.01 ***p < 0.001, n = 3) and time- (B; ***p < 0.001, n = 3) dependent manner. OV2008 cells exhibited higher apoptosis than C13* cells when treated with CDDP. Results are expressed as mean ± SEM (n = 3) and analyzed by 2-way ANOVA and Bonferroni post-hoc test. [**p < 0.01, ***p < 0.001, (versus CDDP = 0; A) and (versus time = 0; B); n = 3]. Figure S3. p-p53 (ser15) interacts with Phb1 and Bak in response to CDDP in chemosensitive CECA cells, but not in chemoresistant cells. (A) OV2008 and C13* cells were treated with CDDP (0–10 μM, 6 h). Protein contents of Phb1, p-p53 (ser15), p-p53 (ser20), Bak and GAPDH were examined by Western blot. Protein-protein interaction was determined by IP-Western. (B) Cell lysates were immunoprecipitated with IgG (control; lanes 1) or Bak antibody. Bak immunoprecipitates were immunoblotted [IP: anti-Bak, WB: anti-Bak, −Phb1, −p-p53 (ser15 and ser20)]. Results show representative images from 3 independent experiments. Results are expressed as mean ± SEM (n = 3) and analyzed by 2-way ANOVA and Bonferroni post-hoc test. [A and B; **p < 0.01, ***p < 0.001 (compared to DMSO), n = 3]. Figure S4. Prolonged CDDP treatment induced mitochondrial localization of Phb1 in CECA cells. (A) OV2008 and (B) C13* CECA cells were cultured with CDDP (0, 10 μM, 0, 3, 6, and 24 h; DMSO as a vehicle), and were examined by confocal microscopy. Cellular localization of Phb1 (Red) and p-p53 (ser15) were shown in representative images. Green: Mitochondrial Marker and Blue: Nucleus marker (DAPI) Merge 1 indicates the merged image between Phb1 and p-p53 (ser15) whereas merge 2 indicates merged image between DAPI and Phb1.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,002 | 0,020 |
| Méta-épidémiologie (sens strict) | 0,002 | 0,001 |
| Méta-épidémiologie (sens large) | 0,002 | 0,001 |
| Bibliométrie | 0,002 | 0,004 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,002 | 0,002 |
| Science ouverte | 0,002 | 0,001 |
| Intégrité de la recherche | 0,002 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,883 | 0,156 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; l’étiquette directe de Gemma et le classifieur distillé Codex s’accordent sur ce qui est montré ici.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».