Additional file 1 of IL-4Rα blockade reduces influenza-associated morbidity in a murine model of allergic asthma
Notice bibliographique
Résumé
Additional file 1: Table E1. Primers used for qPCR assays. Figure E1. Lung Tissue Sectioning. The left lung was cut in a transverse manner to create a superior and an inferior segment. The inferior segment was embedded into paraffin, while the superior segment was further cut in a sagittal manner and embedded. Figure E2. House dust mite (HDM) sensitization delays viral clearance. Phosphate-buffered saline (PBS)-exposed or HDM-sensitized mice were infected with pandemic H1N1 (pH1N1) and sacrificed on days 0, 2, 4, 6, and 8 post-infection. (A) IFN-β protein expression was determined using ELISA on the supernatant of bronchoalveolar lavage fluid (BALF). (B) qPCR was performed on RNA from homogenized lung tissue to determine the number of viral RNA copies, and results were compared using a two-tailed Student’s t-test. Data (n=5 per group per day) were compared using a two-way ANOVA with Sidak’s multiple comparison’s test and are expressed as mean ± SEM. *P<0.05 after correction for multiple comparisons; ns: not significant. Figure E3. House dust mite (HDM) sensitization dampens the induction of interferon stimulated genes (ISGs). Phosphate-buffered saline (PBS)-exposed or HDM-sensitized mice were infected with pandemic H1N1 (pH1N1) and sacrificed on days 0, 2, 4, 6, and 8 post-infection. qPCR was performed on RNA from homogenized lung tissue to measure the gene expression of the interferon stimulated genes (A) MX1, (B) IFITM3, (C) OAS1, (D) ISG15, (E) Viperin, and (F) RIG-I. Results were normalized to expression on day 0 and were compared using a Mann-Whitney U test. Data (n=5 per group per day) are expressed as mean ± SEM. *P<0.05 after correction for multiple comparisons; **p<0.01 after correction for multiple comparisons; ns: not significant. Figure E4. House dust mite (HDM)-sensitized mice present elevated IL-33 and lower IFN-γ levels following pandemic H1N1 (pH1N1) infection. Phosphate-buffered saline (PBS)-exposed or HDM-sensitized mice were infected with pandemic H1N1 (pH1N1) and sacrificed through a time-course study. (A) IL-33 and (B) IFN-γ protein levels were measured using ELISA and multiplex assay, respectively (n=5 per group per day). Data were compared using two-way ANOVA with Sidak’s multiple comparison’s test and are expressed as mean ± SEM. *P<0.05 after correction for multiple comparisons; ** P<0.01 after correction for multiple comparisons; ns: not significant. Figure E5. Bronchoalveolar lavage fluid (BALF) cell differential analysis. BALF was obtained on day 8 post-infection from pandemic H1N1 (pH1N1)-infected mice. The percentage of (A-C) neutrophils, (D-F) lymphocytes, and (G-I) macrophages was determined in a total of 200 counted cells. (J-L) Trypan blue was used on the re-suspended BALF pellet and the absolute number of inflammatory cells in BALF was determined using a hemocytometer. Data were compared using a two-tailed Student’s t-test and are expressed as mean ± SEM. Blue/pink: intranasal phosphate-buffered saline (PBS) (n=10) / intranasal house dust mite extract (HDM) (n=9); green/orange: intranasal HDM followed by prophylactic strategy of IL-4Rα blockade (n=12) / IgG (n=12); black/red: intranasal HDM followed by therapeutic strategy of IL-4Rα blockade (n=12) / IgG (n=10). ** P<0.01; ***P<0.001; ns: not significant. Figure E6. Systemic blockade of Interleukin-4 receptor alpha (IL-4Rα) reduces IL-6 and IP-10 levels in bronchoalveolar lavage fluid (BALF). BALF was obtained on day 8 post-infection from pandemic H1N1 (pH1N1)-infected mice. (A-C) IL-6 and (D-F) IP-10 protein levels were measured in BALF by multiplex assay. Data were compared using the Mann-Whitney U test and are expressed as mean ± SEM. Blue/pink: intranasal phosphate-buffered saline (PBS) (n=10) / intranasal house dust mite extract (HDM) (n=9); green/orange: intranasal HDM followed by prophylactic strategy of IL-4Rα blockade (n=13) / IgG (n=12); black/red: intranasal HDM followed by therapeutic strategy of IL-4Rα blockade (n=12) / IgG (n=12). * P<0.05; ** P<0.01; ns: not significant. Figure E7. HDM-sensitization Induces Immune Cell Infiltration to the Airways. (A) PBS-exposed or HDM-sensitized mice were sacrificed on day 8 following pH1N1 infection or CAF exposure (n=10 except for HDM + pH1N1 n=9). Paraffin-embedded lung tissue obtained from these mice was sectioned and stained with Periodic Acid Schiff (PAS). Fractional lymphoid area was quantified and is expressed as a percentage of lymphoid tissue area per total cross sectional area. Data are expressed as mean ± SEM by one-way ANOVA. (B) PBS-exposed or HDM-sensitized nice were sacrificed through a time-course study (n=5 per group per day). The lungs were homogenized with PBS, and the supernatant was collected for protein analysis using the BCA assay). Data are expressed as mean ± SEM by a two-way ANOVA with Sidak’s multiple comparisons test. ** P<0.01; *** P<0.001.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,003 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,874 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».