Introgression of Clubroot Resistance from Brassica oleracea into B. napus Canola through Interspecific Cross
Notice bibliographique
Résumé
Clubroot disease caused by Plasmodiophora brassicae Woronin is a serious threat to canola production in Canada. Most of the available clubroot-resistant canola cultivars carry major resistance genes of the Brassica A genome. However, the ineffectiveness of this type of resistance has been reported in Canada. On the other hand, clubroot resistance of the C genome of B. oleracea is under quantitative genetic control; this type of resistance is expected to provide a durable resistance in canola. The objective of this thesis research was to investigate the prospects of introgression of the C-genome resistance from B. oleracea into B. napus canola through interspecific hybridization between these two species. For this, B. napus × B. oleracea var. acephala (clubroot resistant) interspecific hybrids were produced through application of in vitro ovule culture technique. Following this approach, 12 hybrids from a total of 175 pollinations were obtained. The F1 plants were self-pollinated for F2 seeds and backcrossed to the B. napus parent for BC1 seeds, and the F2 and BC1 populations were self-pollinated to produce F3 and BC1F2 populations. The F1 plants exhibited high sterility and produced only 0.168 seeds per self-pollination and 0.064 seeds per cross-pollination with the B. napus parent. The F2 and BC1 populations were evaluated for agronomic and seed quality traits. These two populations required around 70 and 40 days more time on average to flower as compared with the B. napus parent, and plants flowering earlier than the B. napus parent could be found in these populations. The average erucic acid in F3 and BC1F2 seeds, harvested from the F2 and BC1 plants, was 13.4 and 6.8%, and glucosinolate content was 37.4 and 30.3 μmol/g seed, respectively. Nevertheless, zero erucic acid and low glucosinolate plants could be obtained from both populations. Plants exhibiting resistance to pathotype 3H could be found in F3 and F4, but not in the BC1F2 population. Several F3 plants had nuclear DNA content similar to the B. napus parent. In addition to the above-mentioned populations, different advanced generation populations of B. napus × B. oleracea vars. capitata and gemmifera (clubroot resistant) interspecific crosses were also evaluated for resistance to pathotypes 3H and 3A. Selection for resistant plants and self-pollination of the selected plants was carried out up to F9 generation, from where a clubroot resistant line was achieved from B. napus × B. oleracea var. capitata cv. Bindsachsener interspecific cross. B. oleracea-specific simple-sequence repeat (SSR) marker alleles could be detected in the clubroot-resistant F8 plants of this cross. However, association between the marker alleles and clubroot resistance could not be established. The results from this thesis research demonstrated the prospects of developing clubroot resistant canola lines from the B. napus × B. oleracea interspecific crosses.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,001 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,012 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».