LuminoTox as a tool to monitor contaminants of emerging concern in municipal secondary effluent and their removal during treatment by ozone
Notice bibliographique
Résumé
Conventional wastewater treatment plants were not designed to remove contaminants of emerging concern (CECs), and hence these chemicals have been shown to contribute to contamination into the environment, where CECs ultimately exist in the parts per trillion to parts per million concentration range. CECs have been shown to induce toxicity in aquatic life which has led to concern from researchers, governments and more recently the general public. Consequently, there is a pressing need for technologies to remove CECs and their associated toxicity, and for wastewater quality measurement methods to monitor these contaminants. Treatment of wastewater by ozone has been shown to reduce or remove many CECs; while many studies have demonstrated a decrease in the toxicity associated with their removal, some have reported a toxicity increase. This highlights the need to monitor the success of wastewater treatment by ozone using bioassays. The LuminoTox bioassay, which measures photosynthetic inhibition, was proposed as a tool for this application. While the LuminoTox has been used for different types of water analysis, there is limited research on its applicability for wastewater monitoring, and in particular, in municipal secondary effluents (SEs). In this PhD project, the LuminoTox was explored as a tool for the detection of CECs in wastewater and for monitoring the removal of CEC-associated toxicity during treatment by ozone. Two current LuminoTox biosensors were explored: Photosynthetic Enzyme Complexes (PECs), and Stabilized Aqueous Photosynthetic Systems I (SAPS I), as well as a new biosensor, SAPS II. In this PhD, the LuminoTox proved to be a good monitoring tool for toxicity of SEs and demonstrated the ability to detect and distinguish changes in CECs in wastewater mixtures. Furthermore, it proved to be excellent at monitoring wastewater during treatment by ozone. The LuminoTox, however, demonstrated limited CEC sensitivity at environmentally relevant concentrations. The LuminoTox pre-concentration method increased the sensitivity of the LuminoTox into the range applicable to native CECs in SE but further development of the sample preparation method is required prior to implementing the technology for wastewater monitoring.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,001 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».