Effects of Heat Stress on HSP70, Sarcolipin, and Phospholamban Content and SERCA Function in Female and Male Rat Skeletal Muscle
Notice bibliographique
Résumé
Regulation of cytosolic calcium (Ca2+) levels in muscle is vital to the health and function of muscle due to its involvement in various signaling pathways and cell functions. Sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) pumps are found on the membrane of the sarcoplasmic reticulum (SR) and function to actively transport Ca2+ from the cytosol into the SR. Previous work has shown that in the face of heat stress (HS), a model for oxidative stress, heat shock protein 70 (Hsp70) expression is induced to protect the cytosolic domain and function of SERCA. Having previously found that, sarcolipin (SLN) and phospholamban (PLN), which regulate SERCA in the transmembrane (TM) region of the pump, can also preserve SERCA function using both in-vitro and ex-vivo HS models we wanted to assess if they can be stress-induced proteins like Hsp70. The purpose of this project was to assess the sex differences in basal protein expression of Hsp70, SLN, and PLN and the effect of in-vivo heat stress on SERCA function, total protein nitrosylation status, and gene and protein time-course expression pattern of the three proteins in male and female rat soleus and white gastrocnemius (WG) muscles. The heat stress protocol involved submerging the lower limbs of the animals either in a 37ºC (control) or 42-42.5ºC (HS) water bath to either maintain core temperature at 37ºC (control) or between 41-41.5ºC for 30 mins and were either allowed no recovery or were allowed to recover for 24 or 48 hours. Analysis of basal expression found that i) Hsp70 gene expression was higher in both muscles in the females, but its protein levels were higher in the male soleus, ii) SLN gene expression was also higher in both muscles in the females, but no sex differences were observed in the protein levels, and iii) PLN protein expression was higher in the male soleus. In-vivo heat stress i) did not affect SR Ca2+ uptake in the solei of both sexes whereas, in the WG, SR Ca2+ uptake was increased after 24 hours in both sexes ii) did not have an effect on the protein nitrosylation status in the soleus of male and female rats and on WG of female rats, iii) induced both gene and protein expression of Hsp70 in the soleus and WG of both male and female rats, iv) induced protein expression of SLN in the male soleus but only gene expression of Sln in the male WG whereas the same effects were not observed in the females and v) did not induce Pln gene expression in either muscle, but did induce PLN protein expression only in the WG of males. The findings from this study show that even with a relatively mild acute stress model like the one used here, both SLN and PLN were upregulated at least in one muscle of males, indicating that these proteins may also function as stress-induced proteins.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».