Regulation of cardiolipin remodeling in hypoglycemia-induced apoptosis
Notice bibliographique
Résumé
Cardiolipin ís a major mitochondrial membrane phospholipid in the mammalian heaÉ and contains a primarily tetra-linoleoyl molecular species composition in this organ.The molecular remodeling of cardiolipin in the heart is essential to maintain this unique fatty acyl composition.The enzyme monolysocardiolipin acyltransferase catalyzes the acylation of monolysocardiolipin to cardiolipin in the molecular remodeling of cardiolipin in mammalian tissues.Monolysocardiolipin has been found to play a crucial role in apoptosis by binding to truncated Bid and causing the mitochondrial release of cytochrome c.Entry of a cell into hypoglycemia-induced apoptosis may be prevented when the cardiolipin molecular composition is maintained, and this maintenance may be mediated by monolysocardiolipin acyltransferase activity.The purpose of this project was to determine whether monolysocardiolipin acyltransferase activity plays a role in preventing hypoglycemia-induced damage to the cardiolipin molecule.Cardiolipin de novo biosynthesis and remodeling were examined in the surviving population of Hgc2 cardiac myoblast cells exposed to metabolic hypoxia.H9c2 cells were induced to undergo apoptosis by addition of 2-deoxyglucose to induce hypoglycemia.H9c2 cells were incubated in the absence or presence of 2-deoxyglucose for 16 hours.Dead cells were removed and the surviving population of cells were incubated with either [1,3-3H]- glycerol or ['l-14C¡-linoleic acid (bound to albumin 1:1 molar ratio) and the radioactivity incorporated into cardiolipin was determined.The cardiolipin pool il size in H9c2 cells treated with 2-deoxyglucose was also determined.In addition, the activities of the cardiolipin biosynthesis and remodeling enzymes, monolysocardiolipin acyltransferase protein and mRNA levels were determined.The [1,3-3H1-glycerol or ¡1-14C¡-linoleic acid incorporated into cardiolipin was unaltered in 2-deoxyglucose-treated cells compared to controls.Then too, the cardiolipin pool size, determined by phosphorous mass, was unaltered in these cells.In addition, the activities of the cardiolipin de novo biosynthetic enzymes were unaltered.Surprisingly, mitochondrial phospholipase Az activity was increased 33% (p<0.05) and monolysocardiolipin acyltransferase activity 1.7-fold (p<0.05) in 2-deoxyglucose{reated cells compared to controls.The increase in monolysocardiolipin acyltransferase activity was due to an elevated expression in monolysocardiolipin acyltransferase protein and this was due to increased mRNA expression.This shows that remodeling/reacylation of cardiolipin was increased in 2-deoxyglucose treated cells.Thus, a compensatory increase in monolysocardiolipin acyltransferase activity and expression occurs in order to maintain the cardiolipin pool size in H9c2 cells exposed to chemically induced hypoxia, in which phospholipase Az activity is elevated.Thus, the increase monolysocardiolipin acyltransferase is protective against accumulation of monolysocardiolipin and apoptosis.ilI
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».