Individuazione di ‘Candidatus phytoplasma ziziphi’ in drupacee in Italia.
Notice bibliographique
Résumé
During the last years in two different areas of northern Italy in which cherry and peach trees are mainly grown it was observed a severe symptomatology that leads plants to decline and die in two-weeks/one month maximum. From the beginning of the summer the affected plants show leaves of smaller size, with chlorosis, reddening, curling aspect, and premature fall; young branches also show some lack of lignifications. Preliminary tests allow to exclude the presence of several biotic or abiotic agents therefore to verify the possible phytoplasma association with the disease, nucleic acid extraction was performed from leaf midribs as well as from phloem scrapes of declining trees. Chloroform/phenol extraction (Prince et al., 1993) was applied before molecular analyses that were then carried out by nested PCR with primers amplifying 16S rDNA, spacer region and part of the 23S region. Samples positive with R16RF2/R2 primers were subjected to RFLP analyses with MseI, RsaI, and HpaII (Lee et al., 1998), restriction profiles obtained from peach and cherry samples were undistinguishable from each others and were identical to the reference sample JWB (jujube witches’ broom) employed as control, therefore the phytoplasmas detected were attributed to ribosomal subgroup 16SrV-B. The further use of CJF1/R1 (Zhu et al., 1977) subgroup 16SrV-B specific primers allow to confirm this identification. Sequencing of R16F2/R2 amplicons obtained from one sample of peach and one of cherry respectively was also performed. After aligning and comparing these sequences with those in GenBank it was possible to determine the presence of 99% homology with sequence AB052877, Candidatus Phytoplasma ziziphi recently described (Jung et al., 2003). \nIt is know from literature that in areas where peach and cherry are mainly cultivated severe phytoplasma diseases often occur, but they are associated with diverse agents: 16SrX-B in Europe, 16SrIII-A in Canada and California, 16rVII-A in Sicily, 16SrV-B in India and China, 16SrXII-A in Italy and Chile. The detection of jujube witches’ broom subgroup phytoplasmas is however reported for the first time in Italy and this must to be taken into consideration for possible epidemic outbreaks. It is important to continue the monitoring at national level in order to verify the real diffusion and spreading of this phytoplasma in Italian cultivations.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,001 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».