Functional assessment of articular cartilage using injury-induced changes in solute transport and improved mechanical characterization
Notice bibliographique
Résumé
Articular cartilage is avascular and comprised mainly of a water-rich extracellular matrix (ECM); thus solute and fluid transport through cartilage ECM are crucial to cartilage physiology. Once injured, cartilage has a limited capacity for self-repair, often leading to long-term degeneration and osteoarthritis due to focal damage. Modern strategies for cartilage repair are promising, however they rely upon detection of tissue damage at an early stage following injury. This thesis explores relationships between cartilage injury, matrix damage, and associated changes in fluid and solute transport through cartilage. Results indicate avenues for improvement upon in vitro and in vivo methods for cartilage functional assessment, and early detection of focal lesions.First, an investigation was performed into the effects of a well-characterized in-vitro model for cartilage injury on the transport properties of a wide range of solutes, including fluorescent solutes and contrast agents. Mechanical injury was associated with a significant increase in effective diffusivity versus uninjured explants for all solutes studied. In contrast, mechanical injury had no effects on effective partition coefficients for most solutes tested. This distinction is important because measurement of equilibrium partitioning underlies many of the methods currently in use for clinical imaging of cartilage, but we found it to be much less sensitive to cartilage injury than non-equilibrium diffusive transport. Our results emphasized enhanced diffusive transport across the articular surface of injured cartilage, which may have important physiological implications for injury and repair situations. These support development of novel non-equilibrium methods for identification of focal cartilage lesions by contrast agent-based clinical imaging. Second, a new methodology for efficient in vitro mechanical characterization of soft tissue and gel samples was developed, which provides some important advantages over existing methods. Using a novel solution to the poroelastic governing equations for creep valid at early times combined with the classical solution for the stress relaxation problem, this method allows for measurement of gel elastic modulus and hydraulic permeability with some inherent redundancy that improves confidence in measured parameters.This thesis therefore emphasizes the development of new approaches for functional assessment of articular cartilage tissue in the laboratory and in the clinic. Improved observation and quantification of solute transport and poroelastic mechanics in cartilage will contribute to improved tissue functional assessment leading to a better understanding of cartilage physiology and more accurate identification of the stages of degenerative disease.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,002 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,001 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,001 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».