Characterization, prevalence, and risk factors of breast myopathies (spaghetti meat, woody breast, white striping) in broiler chickens from Ontario, Canada
Notice bibliographique
Résumé
Spaghetti meat (SM), woody breast (WB), and white striping (WS) are myopathies that affect the pectoral muscle of fast-growing broiler chickens. Although reported in numerous countries with intensive poultry production, no studies have characterized these myopathies in Canada. By obtaining samples from two large processing plants in Ontario, the objectives of this thesis were to 1) determine the prevalence of and identify the risk factors associated with SM, WB, and WS in Ontario broilers; 2) characterize the morphological changes associated with breast myopathies by evaluating the macroscopic and histological features of affected fillets; and 3) evaluate the transcriptomic profiles of normal and SM- or WB-affected fillets using RNA sequencing and droplet digital PCR. \nThe prevalence of SM, severe WB, and mild or moderate WS was 36.3%, 11.8%, and 96.0%, respectively (n = 9,250). Most (85.1%) of the fillets presented with multiple myopathies. Regression analyses showed that the odds of SM increased with live weight and higher environmental temperature during the grow-out period. The odds of WB increased with live weight and flock mortality rate during grow-out. Macroscopically, the odds of SM and severe WB were significantly associated with increased fillet thickness and weight. Histologically, myopathies had overlapping lesions consisting of polyphasic myodegeneration, perivascular inflammatory cuffing, and endomysial accumulation of fibrous tissue and fat. Transcriptomic analysis showed that there were no differences between normal and SM fillets, while the transcriptomic profile of WB fillets significantly differed from both normal and SM. Most of the differentially expressed genes in WB fillets were involved with the extracellular environment and immune response. \nTaken together, our results show that the macroscopic and microscopic features of SM, WB and WS in our cohort were similar to those has been reported from other countries. These myopathies were highly prevalent in Ontario broilers, suggesting a significant economic impact on the Canadian poultry industry. While WS was present in most fillets, risk factor analysis showed that heavier broilers had an increased risk of both SM and severe WB. Lastly, lack of significant differences in expressed genes between normal and SM fillets suggests that SM may not be caused by factors intrinsic to the broiler breast muscle, but rather could be caused by mechanical disruption after slaughter.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».