Diversity of Viral Communities in a Northern Temperate Lake through Metagenomics
Notice bibliographique
Résumé
Viruses in oceanic environments play pivotal roles in regulating nutrient transfer and increasing rates of carbon transport. They have also been implicated in the regulation of micro-organism community dynamics through infection of successful hosts, the stimulation of primary productivity, and the termination of algal blooms. Through auxiliary metabolic genes stolen from host cells, viruses are also thought to influence biogeochemical cycles through gene expression during infection. Viruses have been comparatively well studied in oceanic environments, but there is a dearth of knowledge in their roles in freshwater environments. As toxins produced by harmful algal blooms (HABs) can challenge conventional drinking water operations, it is critical to understand the role viruses may play in their proliferation and potentially their management. Examining viral communities using metagenomics will allow for the characterization of the diversity of viruses and their potential roles in freshwater environments. To accomplish this, metagenomic sequencing was carried out on water samples from Big Turkey Lake (Ontario) over a seasonal period from 2018 to 2020. Multiple viral classifiers were first combined with a viral binning approach, and subsequently generated viral metagenome-assembled genomes (vMAGs) were then evaluated for quality. All contigs identified as viral were assigned taxonomy based on amino acid alignment through BLAST, and the vMAGs were further analyzed through the IMG/VR database to determine if there was notable alignment with viruses that have known hosts. Auxiliary metabolic gene analysis was performed using the KEGG orthology database as a reference. The majority of viral contigs across all samples were unable to be assigned taxonomy, indicating that there is a large amount of unknown diversity of viruses within Big Turkey Lake. Identified contigs were either assigned as bacteriophages from the order Caudovirales or eukaryotic algal viruses from the family Phycodnaviridae, with the presence of virophages observed in low abundance. Bacteriophage abundance remained relatively stable throughout the year with a spike in winter, while eukaryotic algal viruses were most abundant in the summer. Auxiliary metabolic genes related to folate biosynthesis, carbon metabolism, nitrogen fixation, and photosynthesis were identified, and genes related to lipid metabolism were also found during winter. Findings from this study will provide a baseline foundation of viral communities in Big Turkey Lake, allowing for further research into potential hosts for these viruses, and how they could influence the formation and termination of harmful algal blooms in freshwater.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,001 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,001 |
| Bibliométrie | 0,002 | 0,002 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,002 | 0,001 |
| Science ouverte | 0,000 | 0,001 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».