Pre-Hatch Sexing for Chicken Embryo Based on Immuno-Interrogation
Notice bibliographique
Résumé
Intensive selection for commercial poultry production has resulted in highly divergent layer and broiler pedigree lines. However, the sex ratio after hatching remains approximately 1:1, resulting in a large-scale culling of male chicks in the industrial egg-laying sector, which raises concerns about animal welfare and ethics. Therefore, early (pre-hatch) sex identification is considered to be essential to address ethical / animal welfare issues, and the Canadian egg production industry aims to discontinue the practice of killing male chicks after hatching. Determining the sex of the chick embryo using non-invasive or minimally invasive approaches before hatching is the focus of intensive research. In birds, males possess the ZZ genotype, and females the ZW genotype; therefore, sex is determined by the presence of the W sex chromosome. The detection of W chromosome–encoded protein(s) in embryonic tissues may allow for sex prediction. We hypothesized that W-encoded proteins may be detectable in the chorioallantoic membrane (CAM), an extraembryonic tissue, using immunochemical approaches. The objective of this research project was to develop and validate an innovative immunochemical approach for sex determination in-ovo by detecting the presence of CAM protein(s) encoded on the W chromosome at an early embryonic day (ED 6, 8, or 10). In this study, various CAM manipulation techniques were identified and evaluated, including the use of eggshell (ES) surface disinfectants, ES drilling, various surgical tools and sealant methods. My results indicated that CAM at ED 6 is more challenging to sample, with lower retention of embryo viability (assessed at ED 16) than at ED 8 and 10, which are more compatible with embryo viability. LC/MS/MS-based proteomics was utilized to evaluate the protein profile of CAM tissue harvested at embryonic days (ED) 6, 8, 10, and 12, in order to highlight the expression of distinct CAM functionalities during embryonic development and to gain insight into possible sex-based differences. A total of 2,688 CAM proteins were identified, with 2,347, 2,265, 2,351, and 1,267 proteins detected at ED 6, 8, 10, and 12, respectively. Notably, 1,191 common proteins were identified across all stages, while 124, 47, 86, and 2 proteins were uniquely expressed at ED 6, 8, 10, and 12, respectively. Notably, a sex-specific analysis identified 614, 320, 314, and 212 proteins that were uniquely expressed in female embryos, and 212, 273, 144, and 56 proteins only in male embryos at ED 6, 8, 10, and 12, respectively. The project's future focus will be to develop and utilize sensitive, specific polyclonal antibodies against synthetic peptides for W-encoded protein(s), with the goal of optimizing an immunoassay for one or more candidate W chromosome-linked proteins in CAM tissue. These findings highlight the potential of CAM in both research and biomedical applications, as well as providing a basis for the development of in-ovo sexing technology.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».