An Investigation of Seed Longevity and Antioxidant Changes of Conserved Barley Germplasm
Notice bibliographique
Résumé
Seed longevity is crucial for the sustainable ex situ conservation of germplasm in genebanks, yet its reliable assessment remains a significant challenge. This research was conducted to predict the longevity of a diverse barley (Hordeum vulgare L.) germplasm collection comprising cultivated and wild types preserved in long-term storage (LTS) at Plant Gene Resources of Canada (PGRC) for 5 to 37 years, and to study the changes in antioxidant levels during storage. In this study, a quadratic random intercept model was applied to viability data to predict longevity as a flexible modelling approach. Seedling lengths, variation in viability and lengths were analyzed in parallel with the viability model as a novel approach to longevity prediction. To account for intra-specific variation, modelling was also performed within several biological subcategories. Additionally, temporal dynamics of antioxidant parameters—total phenolic content (TPC), DPPH radical scavenging assay and total antioxidant capacity by FRAP assay—were evaluated for a subset of covered barley. Barley germplasm remained viable for nearly four decades in the PGRC base collection, suggesting that the first monitoring test could potentially be deferred until at least 35 years after storage. Distinct patterns of viability loss were observed among covered cultivars, hulless barley and covered wild barley, although the latter two groups were underrepresented. Models based on radicle length and on variability in viability (tCVV) and radicle length (tCVRL), aligned with the viability-based curve for all barley. However, for all covered barley and covered breeding lines, radicle length declined faster than viability, suggesting its potential use as an early indicator of viability loss. Together, these alternative modelling approaches reinforce the longevity estimates derived from the viability model. Changes in antioxidant parameters did not consistently reflect the observed patterns of viability loss across three covered barley germplasm types, although antioxidant parameters were highly correlated with each other (r > 0.7; p > 0.001). Interestingly, covered wild barley showed the highest TPC, DPPH and FRAP activities relative to cultivated and breeding barley groups, which increased with prolonged storage—except for notably low values in the intermediate group. Collectively, this study revealed characteristic patterns of viability loss and differences in antioxidant dynamics among barley subgroups under LTS at PGRC, suggesting that defining longevity parameters at the intra-specific levels may improve the management of seed collections in genebanks.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».