Profiling of <i>Burkholderia pseudomallei</i> variants derived from Queensland’s clinical isolates
Notice bibliographique
Résumé
ABSTRACT Burkholderia pseudomallei ( Bp ), an environmental bacterium and opportunistic pathogen endemic to tropical regions, is highly adaptive and thrives in diverse environments, from soil to human hosts. Bacterial adaptation is critical for survival, virulence modulation, and persistence during infection and can manifest as colony morphotype variation (CMV). Although Bp adaptation has been studied, CMV remains poorly understood. Here, we characterized five clinical Bp isolates exhibiting heterogeneous populations with rough and smooth colony morphologies. We used phenotypic assays, whole-genome sequencing, and proteomics to investigate the molecular pathways reflecting CMV, by comparing smooth and rough morphotypes. Although phenotypic differences in protease activity, hemolysis, mucoidy, iron uptake, and antibiotic sensitivity—including to antimicrobial agents commonly used to treat infections—were rare, these traits alone could not distinguish morphotypes or groups of isolates. Genomic comparisons revealed either no differences or limited isolate-specific mutations, which do not explain the overall difference in phenotypes. In contrast, proteomic analysis uncovered consistent shifts in protein abundance related to virulence, including quorum sensing, DNA methylation, and secretion systems. Rough variants showed higher abundance of EPS-associated proteins, the BpsI3/R3 quorum-sensing system, and the global regulator ScmR, whereas smooth variants displayed higher abundances of proteins belonging to type III/VI secretion and siderophore biosynthesis pathways. These findings suggest that CMV is driven by phase variation and regulatory mechanisms rather than punctual genomic modifications. Our study underscores the limitations of phenotype or genome-based classification alone in the context of CMV and highlights the value of integrated multi-omics approaches to uncover CMV-associated biomarkers, with potential applications in diagnostics and the development of targeted therapies against persistent and drug-resistant Bp infections. IMPORTANCE Burkholderia pseudomallei ( Bp ), the causative agent of melioidosis, is endemic to Australia, Asia, Africa, and the Americas. It predominantly affects Indigenous populations and individuals suffering from diabetes, chronic lung or kidney disease, or alcoholism. Bp is known for its exceptional genomic and phenotypic plasticity, enabling rapid adaptation to diverse environments. This adaptability is reflected by colony morphotype variation (CMV), including reversible phase variation between smooth and rough colonies. In this study, we report rough and smooth colonies from clinical samples and emphasize the importance of characterizing CMV through multi-omics approaches rather than relying solely on genomics and phenotypic traits. By integrating genomic, phenotypic, and proteomic data, we identified that a limited number of mutations, including one in a putative regulatory element, likely drive major molecular changes between morphotypes. These affect the expression of quorum-sensing systems, the transcriptional regulator ScmR, DNA methyltransferase, and virulence-associated genes.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».