Fabrication and characterization of single-strand chitosan fibers for chitosan hydrogel scaffolds
Notice bibliographique
Résumé
Human voice is produced by self-oscillations of the vocal folds that consist two lips of soft tissue located within the larynx. According to the National Institute on Deafness and Other Communication Disorders (NIDCD), around 7.5 million people in the United States have difficulties for using their voices. Smoking, abuse of alcohol and caffeine, chemical exposure, laryngeal cancer, upper respiratory infection, and voice misuse are among patterns of risks that might lead to voice disorders. One common type of voice loss is vocal fold lamina propria (VFLP) scarring disorder. Injectable hydrogels (a gel composed of one or multiple polymers suspended in water) are proposed to potentially recover the elasticity of scarred VFLP. A biomimetic hydrogel based on glycol-chitosan is presently under investigation in the Biomechanics laboratory in the department of mechanical engineering at McGill University in order to treat VFLP scarring disorder. Cells within the chitosan scaffold tend to adopt a spherical shape, which hampers their mobility and movement. We hypothesized that the presence of fibers within the gel, make it possible for the cells to attach and spread along the fibers and migrate. Therefore, our objective in the study outlined in this thesis was to fabricate and introduce single-strand chitosan fibers into the chitosan hydrogel matrix to promote the migration and mobility of fibroblast cells within the composite gel. In order to fabricate the fiber, a mixture of chitosan and acetic acid was prepared. The fibers were fabricated via direct extrusion. The primary solution was injected into a bath of acetone using a syringe pump. Fibers with an average diameter of 50 µm were obtained through selection of a proper needle size. The viability and migration rate of human vocal fold fibroblasts (hVFF) were investigated to study the reaction of the cells to the presence of the fibers. The ultimate strengths of the fibers were quantified with performing tensile tests. The composition and the surface topography of the fibers were studied using a scanning electron microscope (SEM). Swelling tests were performed to quantify the fibers' capacity to incorporate growth factors and drugs. The storage modulus of the composite hydrogels was measured using a torsional rheometer. Overall, the chitosan fibers were successfully fabricated and introduced to the hydrogel and the mechanical and biological properties of the hydrogel scaffold were enhanced significantly. The viability and migration tests confirmed that adding the fibers to the scaffold is a promising method to promote cell migration. The mechanical properties of the composite scaffold were enhanced as expected.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».