Anti‐proliferate Effects of a Marine Macrolide on Cell Cycle Kinetics, Apoptosis, and Gene Expression in LNCaP and PC‐3 Cells
Bibliographic record
Abstract
Iejimalide B (IejB) is a marine macrolide produced by Eudistoma cf. rigida, a sea slug living in the waters near the Japanese island, Ie Jima. We are investigating the effects of IejB on cell cycle kinetics and apoptosis, as well as its effect on gene expression in the prostate cancer cell lines, LNCaP and PC‐3 cells. IejB has a dose and time dependent effect on cell number in both PC‐3 and LNCaP cells. In p53‐/AR‐ PC‐3 cells, IejB induces a G1/S phase arrest at 24 h and causes a transition to S phase arrest at later time points. In comparison, IejB induces G0/G1 phase arrest and apoptosis (Apo‐BrdU staining) in the p53+/AR+ LNCaP cells. These data suggest that IejB exerts its effects by initially inhibiting the cell cycle in G0/G1 and subsequently induces S phase arrest by inhibiting DNA synthesis. Since the induction of apoptosis in LNCaP cells occurs without entry into S phase, IejB appears to have two targets within the cell: one at the bifurcation of the cell cycle and apoptotic pathways, and one that inhibits DNA synthesis. Using RT‐PCR we have compared the effects of IejB on mRNA state levels for 50 genes related to cell cycle and apoptosis. The expression profiles in LNCaP and PC‐3 cells are very similar and dose dependent, suggests that the effects of Iejimalide B on transcription are neither p53 nor AR mediated. (Supported by the Coleman Foundation and the Walther Cancer Institute)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".