Bibliographic record
Abstract
采用RACE—PCR的技术克隆了播娘蒿的油酸脱氢酶基因（DsFAD6），它是催化油酸转化为亚油酸的一个关键酶基因。DsFAD6cDNA全长序列的开放阅读框长1344bp，编码一个由447个氨基酸残基组成的蛋白质，推测的蛋白质相对分子质量为51．16kD，等电点为9．27。序列分析表明DsFAD6的氨基酸序列具有3个高度保守的组氨酸盒子，该结构在去饱和酶中是高度保守的，且在N端预测到信号肽，初步认定该酶定位于质体。序列比对和系统发生分析显示DsFAD6与十字花科植物FAD6基因具有较高一致性。RT-PCR分析表明，DsFAD6在根、茎、叶、花蕾、花及幼嫩角果中均表达，但在茎、叶和幼嫩角果中表达较高。胁迫诱导表达中，DsFAD6在播娘蒿叶片中表达明显受伤害胁迫诱导，但在冷胁迫下呈负相关。
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.006 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".