Intestinal transcellular sodium and phosphate co‐transport activity contributes little to whole body phosphorus homeostasis in the pig
Bibliographic record
Abstract
This study was conducted to examine the expression of the apical Na + ‐phosphate (Pi) co‐transport kinetics and the capacity along the intestinal longitudinal axis and the contribution of this whole gut uptake capacity to whole body phosphorus (P) homeostasis in the pig. The apical membrane vesicles were prepared from the intestinal segmental samples of the duodenum, the proximal and the distal jejunum, the distal ileum, the cecum, and the proximal colon harvested from six weaned pigs (11 kg BW) by Mg 2+ ‐precipitation and differential centrifugation procedures. Initial rates and kinetics of [ 32 P]Pi were measured by the fast filtration in the membrane vesicles at 37°C with membrane potential clamped by SCN ‐ . There were no differences (P>0.05) in the apical + ‐Pi co‐transport maximal activity (Jmax: 1.02 – 2.16 pmol Pi mg −1 protein.s −1 , n = 11) and the transport affinity (Km: 0.034 – 0.086 mM, n = 11) along the intestinal longitudinal axis. The intestinal apical transcellular Na + ‐Pi co‐transport capacity (1.06 ± 0.01 mg Pi kg −1 BW.d −1 , n = 6) only accounted for about 0.44% of the daily bio‐available P requirement (0.24 g P kg −1 BW.d −1 ) of the weanling pig as recommended by NRC (1998). In conclusion, the apical Na + ‐Pi co‐transport activities necessary for absorbing luminal phosphates to maintain the intestinal epithelial metabolism are expressed along the entire small and the large intestinal longitudinal axes. However, the whole gut apical transcellular Na + ‐Pi co‐transport capacity contributes little to the whole body P requirement and homeostasis. Supported by OMAFRA and NSERC of Canada.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".