Abstract 1604: Improved Cardiac Function after Expression of the Human Elastin Gene in the Infarcted Myocardium
Bibliographic record
Abstract
Rationale: After a myocardial infarction, necrosed cardiomyocytes are replaced by fibrotic tissue. Subsequent thinning and expansion of this non-elastic scar contributes to cardiac dilatation and congestive heart failure. We attempted to restore the elastic properties of the scar by implanting the infarct area with cells expressing elastin. Methods: Full-length elastin cDNA was generated by PCR using the human lung cDNA library, and then transfected (in adenoviral vector) into rat bone marrow stromal cells (BMSCs). Western blot and immuno-staining confirmed expression of elastin protein. Seven days after coronary artery ligation, BMSCs transfected with genes for either adeno-elastin (BMSC+Elastin) or adeno-green fluorescence protein (BMSC), or media (control group) were injected into the infarct area of adult rats (n=8/group). Results: Over 8 weeks following cell or media implantation, cardiac function (fractional shortening by echocardiography), ventricular volumes, and load independent indices of cardiac function (end systolic and diastolic volumes, preload recruitable stroke work and end systolic elastance by pressure-volume catheter), were all significantly better preserved in both BMSC groups (p<0.05 vs. media), with further improvements in the BMSC+Elastin group (p<0.05 vs. BMSC group). Biochemical analysis detected over-expression of recombinant elastin, and histological examination revealed that collagen fiber length and diameter were preserved in the scar tissue. Myocardial birefringence determined by polarized light (linear retardance of the tissue structure) was significantly highest in the infarct area of the BMSC+Elastin group (p<0.05 compared to BMSC and media groups). Elastin over-expression by cell-based gene therapy restored the elastic matrix structure in the scar tissue, stabilized the infarct and prevented ventricular dilatation, producing the smallest (p<0.05) scar size and surface area in the BMSC+Elastin group. Conclusions: Over-expression of elastin in transplanted BMSCs contributed to the alignment and organization of an elastic extracellular matrix structure, which prevented cardiac dilatation and dysfunction. Elastin plays a key role in the remodeling of the extracellular matrix.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".