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Record W1151818151

农杆菌介导柱型苹果“鲁加6号”遗传转化体系的建立

2009· article· zh· W1151818151 on OpenAlexvenueno aff
梁美霞, 祝军, 戴洪义

Bibliographic record

Venue分子植物育种 · 2009
Typearticle
Languagezh
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant tissue culture and regeneration
Canadian institutionsnot available
Fundersnot available
KeywordsComputer science
DOInot available

Abstract

fetched live from OpenAlex

本研究以柱型苹果"鲁加6号"试管苗叶片外植体为转化材料,以卡那霉素抗性基因为选择标记,建立了根癌农杆菌介导的柱型苹果"鲁加6号"苹果的遗传转化体系。研究结果表明:在MS+6-BA4.0mg/L+NAA0.05mg/L培养基上,叶片不定芽分化阶段Km的选择压力为20mg/L,不定芽生根阶段Km的选择压力为15mg/L,脱菌培养阶段头孢霉素的适宜浓度为300mg/L。在农杆菌介导的遗传转化过程中,外植体经过36h预培养,侵染10min,共培养48h时,有利于提高遗传转化效率,获得的抗性愈伤组织和抗性芽数均最高。获得的Km抗性植株经PCR检测,扩增出特异条带,初步证明目的基因已整合到苹果基因组DNA中。

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.687
Threshold uncertainty score0.949

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.237
Teacher spread0.228 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

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