Bibliographic record
Abstract
盐分胁迫严重影响植物生长和产量。为了研究木本植物对盐分的适应性，利用5＇RACE技术，从刺槐中克隆得到液泡Na^＋／H^＋逆向转运蛋白基因RpNHX1。该基因长度为2281bp，含有一个开放阅读框，编码535氨基酸残基组成的蛋白，该蛋白序列与大豆GmNHX1和拟南芥AtNHX1相似，氨基酸的同源性分别达85％和69％，RpNHX1属于NHX亚族的NHX-I分枝。用生物信息学的方法预测RpNHX1具有所有Na^＋／H^＋逆向转运蛋白共同的结构特点，即含有疏水的N末端，10个跨膜螺旋区域和一个具有调节功能的C端亲水区域，其中氨氯吡嗪咪敏感基序和CaM结合域也是保守的。在4和5的螺旋区域之间，存在有一串带负电荷氨基酸，显示出有规律的排列模式，这些模式在生物界中的Na^＋／H^＋转运蛋白中是保守的。结合亲疏水资料，我们认为Na^＋／H^＋转运通道结构可能存在于这一区域。另外，也分析了RpNHX1可能的糖基化、酰基化和磷酸化位点。从这些数据中可以看出，RpNHX1可能在细胞中起到Na^＋区隔化作用。
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.002 | 0.003 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.010 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".