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Record W1202771871

棉花环丙烷脂肪酸合酶基因 CPA-FAS-2 启动子的克隆以及特征分析

2013· article· zh· W1202771871 on OpenAlexvenueno aff
杨郁文, 张保龙, 方先文, 陈天子, 刘廷利, Wei Zhu

Bibliographic record

Venue分子植物育种 · 2013
Typearticle
Languagezh
FieldBiochemistry, Genetics and Molecular Biology
TopicLipid metabolism and biosynthesis
Canadian institutionsnot available
Fundersnot available
KeywordsComputer science
DOInot available

Abstract

fetched live from OpenAlex

含有三碳碳环的环丙烷脂肪酸(CPA.FAs)和环丙烯脂肪酸(CPE—FAs)由于具有特殊的理化性质因而在化工领域有特殊的应用。环丙烷脂肪酸合酶基因负责合成CPA—Fas,植物中尚没有这类基因启动子特征的相关报道。本研究克隆了一个棉花环丙烷脂肪酸合酶基因CPA—FAS一2的启动子区域,对该段区域进行生物信息学分析后发现其含有根、花器官以及种子特异表达元件,还含有磷(P)、硫(S)缺乏、病原菌和干旱等逆境响应元件等。通过构建该启动子序列与GUS基因融合表达载体并转化拟南芥,对T,代转基因材料染色后发现该启动子不仅具有器官表达差异性,并且还受发育时期影响。GUS基因在幼苗期并不表达,只在植株成熟期表达,并且表达部位只限于茎基部、根、种子以及花药。进一步利用GUS定量,结果表明该启动子受P缺失以及干旱胁迫的诱导。本研究进一步明确了环丙烷脂肪酸合酶基因的表达特征,有利于促进该基因在种质创新中的利用。

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.014
Threshold uncertainty score0.035

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.003
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.002
Science and technology studies0.0020.004
Scholarly communication0.0050.006
Open science0.0010.001
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0090.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.207
Teacher spread0.200 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2013
Admission routes1
Has abstractyes

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