INDUCIBLE NITRIC OXIDE SYNTHASE (INOS) IN MURINE PULMONARY MICROVASCULAR ENDOTHELIAL CELLS (PMEC) MODULATES NEUTROPHIL APOPTOSIS.
Bibliographic record
Abstract
Nitric oxide modulates many aspects of PMN function. We investigated the effects of migration across PMEC and PMEC iNOS on PMN apoptosis (Ap) in vitro. PMEC and bone marrow PMN were isolated from iNOS wild type (WT) and knockout (KO) mice. PMN were cultured alone or co‐cultured with PMEC monolayers with or without LPS/IFN‐γ (LI). The Ap was assessed by microscopic changes, annexin‐V (AV) binding by FACS, and caspase‐3 activity (C3A). As well, 24 hr after exposure of WT vs KO mice to intratracheal LI, PMN were collected by BAL and Ap assessed. AV positive PMN (% of total cell) image In non‐migrated PMN, LI increased AV binding. However, there were no differences between WT and KO PMN. In contrast, Ap by AV binding was significantly greater in PMN post‐migration across KO PMEC vs WT PMEC in the presence of LI. Microscopy confirmed these significant differences in PMN Ap. In freshly isolated BAL‐PMN, Ap was similar in PMN from WT and KO mice. However, following a further 24 hr incubation, KO BAL‐PMN had markedly greater Ap vs WT BAL‐PMN (15.2 ± 3.3% vs. 3.0 ± 0.4%, P<0.05). Paradoxically, LI stimulation of PMN decreased C3A by 40–50% in non‐migrated PMN and by 80–90% in trans‐PMEC migrated PMN, independent of PMN or PMEC iNOS. These data suggest that iNOS expressed in PMEC, but not in PMN, has an important anti‐apoptotic effect on PMN during trans‐PMEC migration and this effect seems to be caspase‐3 independent. HSFO/Canada, OTS, LHSC Research Inc
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".