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Record W1208282387

柱型苹果开花抑制基因 FLOWERING LOCUS C 基因的克隆与表达分析

2014· article· zh· W1208282387 on OpenAlexvenueno aff
刘伟, 戴洪义, 张玉刚, 孙晓茜, 刘文莲, 刘更森, 柏素花

Bibliographic record

Venue分子植物育种 · 2014
Typearticle
Languagezh
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant Reproductive Biology
Canadian institutionsnot available
Fundersnot available
KeywordsBusinessComputer science
DOInot available

Abstract

fetched live from OpenAlex

开花抑制基因FLC(FLOWERING LOCUS C)是春化作用中的关键基因,是控制开花时间的枢纽。本研究采用RT-PCR方法,以柱型苹果杂交后代群体中早花株系为试验材料,克隆得到柱型苹果花发育相关基因Md FLC。结果显示,Md FLC开放阅读框长度为603 bp,编码201个氨基酸,推测蛋白质分子量为47.68 k D,等电点为5.18;Md FLC蛋白二级结构包含9个α-螺旋,2个β-折叠区和12个β-转角,具有MADS典型的结构域。系统进化树分析表明,Md FLC与沙梨FLC进化关系最近,与豌豆的亲缘关系最远。半定量PCR及荧光定量PCR分析结果表明,Md FLC基因在普通型苹果‘富士’及柱型苹果株系(95-31,95-41,95-45,95-107,95-152)的花芽和花不同部位(花瓣,花蕊,花萼,花托)均有表达,在柱型苹果花芽、花瓣、花蕊、花萼及花托中的表达量均低于在富士苹果中对应部位的表达量,在花芽中的表达差异尤为显著,暗示了Md FLC基因参与了柱型苹果花芽分化以及开花进程。

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.001
Science and technology studies0.0010.001
Scholarly communication0.0020.001
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0050.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.219
Teacher spread0.213 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

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