KCNQ gene expression in the murine vasculature
Bibliographic record
Abstract
KCNQ2‐5 are considered to be only expressed by neurones. Ohya et al. (Circ Res., 92, –1023, 2003) showed that full length KCNQ1 and a C‐terminal truncated variant were expressed in murine portal vein (mPV), where it contributed to vascular contractility. However, recent studies revealed that murine aorta and various conduit arteries expressed KCNQ4 and KCNQ5 abundantly. The present study used similar techniques that reinvestigated KCNQ channel gene expression in the mPV and analysed further the role of KCNQ expression products (Kv7.1‐5) in the vasculature. Nested PCR experiments revealed that KCNQ genes in addition to Q1 were present in the mPV and this was confirmed by immunocytochemical experiments with antibodies against KCNQ4 expression products. A functional role for ion channels encoded by KCNQ2‐5 was supported by electrophysiological and functional experiments with retigabine, an activator of ion channels encoded by all KCNQ genes except KCNQ1 . Retigabine enhanced outward K + currents and relaxed spontaneous contractions of whole mPV. The KCNQ channel blocker XE991 contracted the aorta, carotid and femoral artery and the degree and rate of contraction was enhanced by contracting the tissue slightly with 0.1 μM phenylepherine. These data support a role for KCNQ channels in defining vascular reactivity. This work was supported by the British Heart Foundation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".