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Record W125914590 · doi:10.1094/asbcj-59-0001

Effect of Cell Surface Hydrophobicity, Charge, and Zymolectin Density on the Flocculation of <i>Saccharomyces Cerevisiae</i>

2001· article· en· W125914590 on OpenAlexafffund
Yu-Lai Jin, L. L. Ritcey, R. Alex Speers, Peter J. Dolphin

Bibliographic record

VenueJournal of the American Society of Brewing Chemists · 2001
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicFungal and yeast genetics research
Canadian institutionsDalhousie University
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsFlocculationYeastSaccharomyces cerevisiaeSurface chargeMannoseChemistryChromatographyFluorescence microscopeCharge densityBiophysicsFluorescenceBiochemistryBiologyOrganic chemistry

Abstract

fetched live from OpenAlex

Cell surface investigations for the yeast strains LCC1209 (Flo1 phenotype), LCC125 (NewFlo phenotype), and LCC1240, a nonflocculent variant of LCC1209, are reported. The strains were examined for their degree of flocculation, surface hydrophobicity, and surface charge. Cell surface zymolectin density was quantitated with a fluorescent avidinfluorescein isothiocyanate probe as well as a radio-labeled tritiated-mannose probe. Additionally, fluorescence microscopy was employed to examine the cell walls of the two flocculent yeast strains. The strains showed different hydrophobicity, surface charges, and apparent zymolectin densities. Cell surface hydrophobicity correlated with flocculation significantly (r = 0.684, P < 0.001). It was found that 82 and 75% of the variations in flocculation (n = 48) can be explained by the level of zymolectin density and zymolectin affinity for the Flo1 and NewFlo strains, respectively.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.260
Teacher spread0.252 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations50
Published2001
Admission routes2
Has abstractyes

Explore more

Same venueJournal of the American Society of Brewing ChemistsSame topicFungal and yeast genetics researchFrench-language works237,207