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Erythromycin and Rho‐Kinase Activity: Another Pathway Responsible for its Gastrointestinal Motility‐Enhancing Effect

2015· article· en· W1421749317 on OpenAlexaff
Jaques Belik, Christopher Welsh, Yulia Shifrin, Jingyi Pan

Bibliographic record

VenueThe FASEB Journal · 2015
Typearticle
Languageen
FieldMedicine
TopicGastrointestinal motility and disorders
Canadian institutionsHospital for Sick Children
Fundersnot available
KeywordsMotilinErythromycinGastric emptyingMotilityInternal medicineEndocrinologyStomachGastrointestinal hormoneBiologyGastrointestinal tractReceptorMedicineNeuropeptideCell biologyBiochemistry

Abstract

fetched live from OpenAlex

Erythromycin enhances gastrointestinal motility and is used to treat feeding intolerance in neonates. Erythromycin is thought to enhance gastric emptying through activation of motilin receptors, but the mechanism is not completely understood and motilin does not induce gastric contraction in newborn rabbits. Hypothesizing that erythromycin promotes gastric emptying via motilin receptor independent pathways, we evaluated its effect on gastric and pyloric tissue. Adult rabbit and newborn rat tissue were studied, since motilin receptors are present in the former, but not in rodents. The in vivo acute erythromycin effect on newborn rodent gastric emptying was measured via ultrasound. Gastrointestinal muscle tone is directly dependent on rho‐kinase II (ROCK) activity, thus the phosphorylated MYPT/total MYPT expression ratio was utilized as a surrogate marker of its activity and measured in fundic and pyloric tissue following carbachol exposure in the absence or presence of erythromycin. Results Erythromycin promoted a similar pattern of ROCK activity changes in both species. Erythromycin exposure significantly increased fundic, but reduced pyloric tissue ROCK activity, thus favoring enhanced gastric emptying. Erythromycin ip injection in newborn rodents increased the 3 hour post‐feed stomach milk clearance from 60±2 % to 76±3 % (P<0.01). Conclusion Erythromycin enhances gastrointestinal motility via rho‐kinase pathway and this explains its effects in newborn rodents where the gastrointestinal tract motilin receptors are absent. image

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.040
GPT teacher head0.283
Teacher spread0.243 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2015
Admission routes1
Has abstractyes

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