Molecular characterization of fowl adenovirus serotype 4 (FAV-4) isolate associated with fowl hydropericardium-hepatitis syndrome in Pakistan.
Bibliographic record
Abstract
A study was conducted to characterize local fowl adenovirus serotype 4 (FAV-4) associated with hydropericardium syndrome (HPS) at molecular level. For this purpose, chicken livers naturally infected with HPS virus were aseptically collected from a field outbreak in commercial broilers from Faisalabad, Pakistan. Virus purification was done using a 15-45% (w/v) sucrose gradient. Virus was confirmed by agar gel precipitation test. Viral DNA was extracted and variable region hexon gene of 0.7 kb in size was amplified by PCR and sequenced. Nucleotide sequence (730 bp) and deduced amino acid sequence (243) of isolate was compared with already published sequences of FAV4. Sequence analysis of the variable region hexon gene confirmed that the virus belonged to avian adenovirus serotype 4. The nucleotide sequence of the Pakistani isolate had a 94% to 98% homology with Indian isolates, 97% homology with Belgium and Russian isolates and 96% homology with Canadian isolate. The amino acid sequences of the Pakistani isolate had an almost 87% to 98% homology with Indian, 92% and 95% homology with Belgian, 96% homology with Russian and 95% homology with Canadian isolate.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".