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Chromatin Immunoprecipitation in Mammalian Cells

2009· article· en· W145266312 on OpenAlexaff
Amy Svotelis, Nicolas Gévry, Luc Gaudreau

Bibliographic record

VenueMethods in molecular biology · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Chromatin Dynamics
Canadian institutionsUniversité de Sherbrooke
Fundersnot available
KeywordsChromatin immunoprecipitationChromatinChIA-PETChIP-on-chipChIP-sequencingBiologyCell biologyBivalent chromatinRNA polymerase IIImmunoprecipitationPromoterGeneTranscription (linguistics)Chromatin remodelingMolecular biologyGene expressionGenetics

Abstract

fetched live from OpenAlex

The ensemble of the genes in the mammalian genome is organized into a structure of DNA and proteins known as chromatin. The control of gene expression by the proteins that bind to chromatin regulates many cell processes, such as differentiation and proliferation. Transcription of protein-encoding genes in mammalian cells is performed by the concerted action of the RNA polymerase II holoenzyme, transcription factors, co-activator complexes that bind to the promoter areas of genes. In addition, different proteins can interact with these complexes and chromatin to create a repressive state. In order to fundamentally understand transcriptional control, it is important to define the areas that these proteins will bind. Classical laboratory techniques unable to provide distinct locations of these factors have now been replaced by the chromatin immunoprecipitation (ChIP) assay. The ChIP technique allows us to isolate chromatin along with its associated proteins from cells and analyse the binding sites of specific proteins and complexes at high resolution.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.002
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.017
Threshold uncertainty score0.056

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.002
Meta-epidemiology (narrow)0.0020.002
Meta-epidemiology (broad)0.0030.003
Bibliometrics0.0030.003
Science and technology studies0.0020.001
Scholarly communication0.0020.001
Open science0.0040.002
Research integrity0.0020.005
Insufficient payload (model declined to judge)0.0170.033

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.331
Teacher spread0.322 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations22
Published2009
Admission routes1
Has abstractyes

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