Effect of Vitamin B <sub>6</sub> ‐antagonist from Flaxseed on Amino Acid Metabolism in Moderately Vitamin B <sub>6</sub> ‐deficient Rats
Bibliographic record
Abstract
Pyridoxal 5‐phosphate (PLP) plays a crucial role as a cofactor in amino acid metabolism. There is a prevalence of moderate vitamin B 6 deficiency in the population that may be exacerbated through the ingestion of 1‐amino D‐proline (1ADP), a B 6 ‐antagonist found in flaxseed. We investigated the nutritional co‐insult induced by 1ADP intake (flax‐derived vs. synthetic) and moderate B 6 deficiency in a rodent model. Male weanling rats (n=8/treatment) consumed a semi‐purified diet containing either 7 (adequate) or 0.7 mg/kg diet (moderately deficient) pyridoxine(PN)•HCl, each with 0 or 10mg/kg diet of 1ADP from synthetic or flaxseed‐derived sources, for 5 weeks. Plasma B 6 vitamers were significantly decreased ( P 蠄0.001) due to 1ADP ingestion, irrespective of source, in moderately B 6 ‐deficient rats. Moderate B 6 ‐deficient rats consuming 1ADP as a flaxseed extract exhibited, relative to controls, a significant increase in plasma cystathionine ( P =0.007), and decreases in plasma α‐aminobutyric acid ( P =0.002) and glutamic acid ( P =0.01) concentrations. However, the synthetic 1ADP elicited greater perturbations in plasma amino acids, compared to the 1ADP extracted from flaxseed. Both 1ADP sources inhibited the activities of the PLP‐dependent cystathionine β‐synthase and cystathionine γ‐lyase ( P <0.001). The metabolic perturbations of 1ADP exposure were not evident in rats consuming adequate B 6 . The current data provide evidence that an anti‐pyridoxine agent now prevalent in the human food supply may pose challenges to those individuals presenting with marginal B 6 status. Supported by the Natural Sciences and Engineering Research Council of Canada (NSERC).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".