Morphological and Physiological Evidence for a Developmental Cycle in <i>Legionella pneumophila</i>
Bibliographic record
Abstract
This chapter focuses on the cyclical nature of some morphological and physiological changes that take place in infected HeLa cells and during growth in vitro. Coverslip cultures of HeLa cells were infected with purified mature intracellular forms (MIFs), and changes in Gimenez staining were followed for up to 3 days. The bacterial strains used throughout these studies were Lp1-SVir and 2064, two virulent strains previously reported and characterized. Cultures with 100% green forms (after Gimenez staining) were identified upon growth of Legionella pneumophila on buffered charcoal-yeast extract (BCYE) plates. Rowbotham reported earlier that infection of amoebae resulted in the formation of small, highly motile, and infectious legionellae that are believed to be equivalent to the HeLa-derived MIFs reported. Purified MIFs were placed in buffered yeast extract (BYE) and their growth was followed through viable cell counts. Researchers concluded that the green forms arising in the BYE cultures were replicating bacteria that developed from the MIFs originally used as inoculum. In summary, L. pneumophila undergoes cyclical morphological and physiological changes that suggest the existence of a developmental program.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.006 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".