Mitochondrial‐Derived Vesicle Formation in Cardiac Mitochondrial Quality Control
Bibliographic record
Abstract
Mitochondrial quality control (QC) is critical for maintenance of efficient cardiac function. Recently, a novel mechanism of mitochondrial QC, acting upstream of autophagy, has been uncovered – mitochondrial derived vesicle (MDV) formation. Our aim was to demonstrate MDV formation in a cardiac model and determine its relevance in mitochondrial pathophysiology. In H9c2 cardioblasts, common mitochondrial stressors (antimycin‐A and H 2 O 2 ) resulted in a significant increase in MDV formation after only 60 min of exposure. The chemotherapeutic doxorubicin (DOX) induced maximal MDV formation after 30 min of treatment, with the levels of MDVs returning to baseline at 24h. In vivo MDV formation and response to stress was analyzed in mice subjected to Sham injection or acute DOX treatment. Despite the absence of echocardiographic differences between groups, mitochondrial functional analysis revealed decreased mitochondrial respiratory capacity, increased ROS production, and susceptibility to PTP opening in the DOX‐treated group. Morphological analysis by TEM revealed a substantial increase in the number of MDVs in the heart of DOX‐treated animals. Our results thus demonstrate that in a cardiac cell model MDV formation is a fast‐response mechanism of mitochondrial QC stimulated by mitochondrial and cellular stressors. Moreover, in addition to the previous reports of in vitro MDV formation, this study demonstrates the existence and responsiveness of MDV formation in cardiac mitochondrial QC.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".