Caracterização biológica, genética e sorológica de uma amostra de vírus da raiva isolada de eqüino de uma região próxima de São Paulo, Sudeste do Brasil
Bibliographic record
Abstract
The biologic behavior of a rabies virus recently isolated from an equine raised at a region neighbor to São Paulo, Southeast Brazil, the isolate M82-02, was studied in mice by inoculating through intracerebral and intramuscular route, for evaluation of characteristics related to its infectivity, pathogenicity, incubation period and course of clinical illness and also the virus capacity to invade other non-nervous tissues.The first intracerebrally passaged mouse brain was submitted for antigenic typing by using a set of monoclonal antibodies (MABs) prepared at the Canadian Food and Inspection Agency, Ottawa, Canada, and it was found to be a variant of vampire-bat related rabies virus.After extraction of genetic material, the sample was characterized genetically at the National Institute of Infectious Diseases, Tokyo, Japan, ant the isolate belonged to genotype 1 of the Lyssavirus gene, closely related to the Vampire-bat related virus group-VRRV, common among the Brazilian rabies virus isolates from herbivores and vampire bat Desmodus rotundus.The successive intracerebral and intramuscular passages in mice provoked a slight increase in the virus titer, and the stabilization of the incubation period.In its 10 th passage, the isolate was used as the antigen in mouse serum neutralization test, for the assessment of equine sera which had been vaccinated with a commercial PV rabies inactivated virus vaccine, the equine sera were tested in parallel with the CVS strain and some sera were found with neutralizing titer >0.5 IU/mL.Using the isolate M82-02 of rabies virus as an antigen in the neutralization test, no significant difference could be detected, when the results were compared to that of the CVS strain.By means of direct fluorescent antibody (dFA) test, the presence of rabies virus antigen was detected in tissues of brain, lung and kidneys of mice inoculated by intramuscular route, especially in serially passaged materials.The CDC-potency test for evaluation of a commercial vaccine using the field isolate M82-02 as a challenge virus showed a poorer result than the challenge with the fixed CVS strain, however, this method needs further modifications for the routine use.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".