Manipulating the Mouse Embryo: A Laboratory Manual
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- Validation status
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Abstract
Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- Topic
- Animal Genetics and Reproduction
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- Toronto Centre for Phenogenomics
- Funders
- —
- Keywords
- TransgenesisBiologyBlastocystEmbryoSomatic cell nuclear transferEmbryonic stem cellGeneticsEmbryo cultureCloning (programming)Chimera (genetics)Cell biologyReproductive technologyCryopreservationGeneEmbryogenesis
- Has abstract in OpenAlex
- yes